Cat. #154102
Immortalised CD40L deficient B Cell Line
Cat. #: 154102
Unit size: 1x10^6 cells / vial
Availability: 3-4 weeks
Organism: Human
Disease: Hyper IgM Syndrome type 1
Model: Immortalised Line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Pranab K Das
Tool Details
*FOR RESEARCH USE ONLY
- Name: Immortalised CD40L deficient B Cell Line
- Alternate name: TRAP, TNFSF5, TNF-Related Activation Protein, T-Cell Antigen Gp39
- Parental cell: B cells from CD40L deficient patient
- Organism: Human
- Disease: Hyper IgM Syndrome type 1
- Growth properties: Maintain at 3x105 to 7x105 cells per ml for optimal growth. Replenish growth medium twice per week
- Model: Immortalised Line
- Conditional: No
- Description: Can be used as antigen presenting cells. Hyper IgM syndromesÄÂ? is a group ofÄÂ? primary immune deficiency disordersÄÂ? characterised by defectiveÄÂ? CD40ÄÂ? signalling;ÄÂ? viaÄÂ? B cellsÄÂ? affectingÄÂ? class switch recombinationÄÂ? (CSR) andÄÂ? somatic hyper mutation.ÄÂ? ImmunoglobulinÄÂ? (Ig) class switch recombination deficiencies are characterised by elevated serumÄÂ? Immunoglobulin MÄÂ? (IgM) levels and a considerable deficienc...
- Production details: B Cells were isolated from PBMCs of CD40L deficient Hyper IgM Syndrome patients using standards methods. B cells were transformed with Epstein-Barr virus.
Handling
- Format: Frozen
- Growth medium: RPMI 1640 medium supplemented with 10% FCS, 10mM HEPES buffer, 50U/ml penicillin and 50 ??g/ml streptomycin
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
