p53Lck-TLX1 Mouse

Tool Details

*FOR RESEARCH USE ONLY

• Tool name: p53Lck-TLX1 Mouse
• Alternate name: T Cell Leukemia Homeobox 1, Homeo Box-11, Proto-Oncogene TCL-3, HOX11, TCL3
• Tool sub type: Mouse
• Disease: T cell acute lymphoblastic leukemia
• Model: Transgenic
• Model description: Transgenic mouse expressing human transcription factor Tlx1 in T cell progenitors under control of the LCK promoter
• Conditional: No
• Description: TLX1 is an orphan homeobox gene. It was originally discovered in association with 4-7% of cases of T cell acute lymphoblastic leukaemias harbouring a t(10;14)(q24;q11) cytogenetic abnormality. Research showed the presence of the TLX1 gene on chromosome 10q24 juxtaposed with the T cell receptor (TCR) delta regulatory elements on chromosome 10, resulting in aberrant over-expression of the full and intact TLX1 gene. Juxtaposition with the TCR alpha locus was also seen. Elevated TLX1 expression in leukemic blasts is also detected in the absence of a translocation in ~50% (37/76) of paediatric T-ALL cases, as well as rare cases of B-AL. Clinically, TLX1+ T-ALLs are associated with a genetic signature associated with maturation arrest at an early cortical thymocyte stage of T cell development. This model organism recapitulates many molecular features of human leukaemias induced by the TLX family of TF oncogenes.
• Genetic background: The human TLX1 cDNA was amplified by PCR using BamHI restriction site containing primers and cDNA of the human T-cell leukemia cell line ALL-SIL as template and was cloned in the pUC1017 vector, downstream of the mouse T-cell specific p56Lck proximal promoter
• Strain: C57BL/6
• Production details: The human TLX1 cDNA was amplified by PCR using BamHI restriction site containing primers and cDNA of the human T-cell leukemia cell line ALL-SIL as template and was cloned in the pUC1017 vector, downstream of the mouse T-cell specific p56Lck proximal promoter
• Additional notes: Transgenic mouse expressing human transcription factor Tlx1 in T cell progenitors under control of the LCK promoter

Handling

• Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

• Target: TLX1

References

• De Keersmaecker et al. 2010. Nat Med. 16(11):1321-7. PMID: 20972433.
• The TLX1 oncogene drives aneuploidy in T cell transformation.