Cat. #153814
Native SDS PAGE Kit
Cat. #: 153814
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This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: University of Wisconsin-Milwaukee
Tool Details
*FOR RESEARCH USE ONLY
- Tool name: Native SDS PAGE Kit
- Molecular formula: Sample Buffer: 100 mM Tris HCl 150 mM Tris Base 0.01875% Coomassie G-250 0.00625% Phenol Red 10% Glycerol pH 8.5 Run Buffer: 50 mM MOPS 50 mM Tris Base 0.0375% SDS pH 7.7
- Description: There is a great need for a robust method that combines the electrophoretic resolution of proteins with retention of their functional behavior. The laboratory of Dr. David Petering has devised a new method of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in which proteins can be well separated during electrophoresis as well as maintain their native 3-dimensional conformations and functional activity. This native SDS-PAGE method will allow for important new experiments t...
- Solubility: N/A
- Additional notes: Can be performed using commercial pre-casted gels. Simple technique and requires only one buffer system. Can retain the functional activity of proteins tested. Can retain binding of protein complexes. The neutral pH of the NSDS buffer system has an advantage over the high pH Tri-glycine system for separation of the pH sensitive proteins.
Handling
- Storage conditions: RT
- Shipping conditions: Dry Ice
References
- Nowakowski et al. 2014. Metallomics. 6(5):1068-78. PMID: 24686569.
- Native SDS-PAGE: high resolution electrophoretic separation of proteins with retention of native properties including bound metal ions.
