Cat. #153793
pTBNde Vector
Cat. #: 153793
Sub-type: pBluescript KS
Availability: Please enquire for quantities and pricing
Bacterial Resistance: Ampicillin
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Prof Emanuele Buratti
Institute: International Centre For Genetic Engineering And Biotechnology (ICGEB)
Tool Details
*FOR RESEARCH USE ONLY
- Tool name: pTBNde Vector
- Tool sub type: pBluescript KS
- Backbone size without insert: 2958
- Bacterial resistance: Ampicillin
- Description: Concentration 1.35mg/ml
- Additional notes: This vector is a typical hybrid minigene that contains a ‘‘simplified’’ version of the gene that will be evaluated for pre-mRNA splicing. In particular, it contains at the 5' end an alfa-globin gene promoter and SV40 enhancer sequences to allow polymerase II transcription in the transfected cell lines. This is followed by a series of exonic and intronic sequences that may derive from a reporter gene or from the gene context itself. In this case, the reporter gene is composed of a-globin and fibronectin exons, while at the 3' end a functionally competent polyadenylation site, derived from the alfa-globin gene, is present. The genomic DNA region of interest that contains a putative splicing mutation is introduced into the minigene in a unique restriction site (NdeI).
Application Details
- Application notes: Concentration 1.35mg/ml
References
- Pagani et al. 2000. J Biol Chem. 275(28):21041-7. PMID: 10766763.
- Splicing factors induce cystic fibrosis transmembrane regulator exon 9 skipping through a nonevolutionary conserved intronic element.
