Anti-mtEF-Tu

Tool Details

*FOR RESEARCH USE ONLY

• Name: Anti-mtEF-Tu
• Alternate name: Mitochondrial Elongation Factor Tu, EF-Tu, P43, Elongation Factor Tu, Mitochondrial, EF-TuMT, COXPD4, EFTU
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: Predicted 43 kDa
• Reactivity: Human
• Host: Mouse
• Application: WB
• Description: Two elongation factors, EF-Tu and EF-Ts, participate in the elongation phase during protein biosynthesis on the human mitoribosome. The mitochondrial EF-Tu promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Mutations identified in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy
• Immunogen: Recombinant protein

Target Details

• Target: Mitochondrial EF-Tu
• Molecular weight: Predicted 43 kDa
• Target background: Two elongation factors, EF-Tu and EF-Ts, participate in the elongation phase during protein biosynthesis on the human mitoribosome. The mitochondrial EF-Tu promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Mutations identified in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy

Applications

• Application: WB

Handling

• Format: Liquid
• Concentration: 0.9-1.1mg/ml
• Unit size: 100 ug
• Storage buffer: Thaw in RPMI + 10% condimed + 20% FCS + 1x pen and Strep + 1 x L-glutamine (~2.7mM). Once growing serially dilute and reduce condimed from 10% to 5% to 2% to 0%. After that can start to drop FCS from 20% to 15% to 10% or even as low as 5%. When growing sufficiently, then stop feeding, when approx. 80% dead cells look shriveled and medium goes yellowish, then pellet the cells down hard and retain the supernatant. This is then filtered to remove as much non-antibody as possible and used as anti-serum.
• Storage conditions: 4° C
• Shipping conditions: Shipping at 4° C