Cat. #153422
H357 Cell Line
Cat. #: 153422
Unit size: 1x10^6 cells / vial
Tissue: Tongue
Disease: Cancer
Model: Tumour line
£575.00
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Contributor
Inventor: Stephen Prime
Institute: University of Bristol
Tool Details
*FOR RESEARCH USE ONLY
- Name: H357 Cell Line
- Tissue: Tongue
- Disease: Cancer
- Growth properties: Adherent
- Model: Tumour line
- Conditional: Yes
- Description: Established from a squamous cell carcinoma (SCC) of the tongue (20mm) of a 74 year-old male patient. STNMP stage I, well differentiated, node negative tumour. Mutant p53, codon 110 exon 4, G to A; the previously reported mutant Ha-ras status of this cell line: codon: 13, G to A, codon 61, A-G is under investigation. This cell line is highly responsive to TGF beta and undergoes epithelial to mesenchymal transition. It expresses high levels of TGF beta1. Fahey et al (1996). Tumourigenic in athymic nude mice on subcutaneous injection and non-tumorigenic on orthotopic injection. Haplotype information: A*02,A*31; B*40,B*44; Cw*03,Cw*05
- Cellosaurus id: CVCL_2462
Target Details
- Target: Human oral squamous cell carcinoma, tongue
Handling
- Format: Frozen
- Growth medium: Split sub-confluent cultures (70-80%), approximately every 5-6 days, 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37?° C. Suggested seeding density 5 x 1000 cells/cm?Â. Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability. DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum ...
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Fahey et al. 1996. Br J Cancer. 74(7):1074-80. PMID: 8855977.
- Dysregulation of autocrine TGF-beta isoform production and ligand responses in human tumour-derived and Ha-ras-transfected keratinocytes and fibroblasts.
- Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.
- Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
- Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893.
- TGF-beta receptor regu...
