H314 Cell Line

Tool Details

*FOR RESEARCH USE ONLY

• Name: H314 Cell Line
• Tissue: Oral Cavity
• Disease: Cancer
• Growth properties: Adherent
• Model: Tumour line
• Conditional: Yes
• Description: Established from a squamous cell carcinoma (SCC) of the floor of the mouth (20mm-40mm) of an 82 year-old male patient. STNMP stage II, moderately differentiated, node positive tumour. Mutant p53, codon 176 exon 5, G to T and 373 exon 11, A to G; wild type K-, N- and Ha-ras. Tumourigenic in athymic nude mice on subcutaneous injection; on injection into the floor of the mouth, cells spread to lymphatics and lymph nodes. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that SSC cell lines can lose their Y chromosome.
• Cellosaurus id: CVCL_2460

Target Details

• Target: Human oral squamous cell carcinoma, floor of mouth

Handling

• Format: Frozen
• Growth medium: Split sub-confluent cultures (70-80%), approximately every 6-7 days, 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37?‚°C. Suggested seeding density 7 x 1000 cells/cm?‹Â›. DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice

References

• Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.
• Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
• Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893.
• TGF-beta receptor regulation mediates the response to exogenous ligand but is independent of the degree of cellular differentiation in human oral keratinocytes.
• Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.
• Epidermal growth f...