Cat. #153420
H157 Cell Line
Cat. #: 153420
Unit size: 1x10^6 cells / vial
Tissue: Buccal mucosa
Disease: Cancer
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Stephen Prime
Institute: University of Bristol
Tool Details
*FOR RESEARCH USE ONLY
- Name: H157 Cell Line
- Tissue: Buccal mucosa
- Disease: Cancer
- Growth properties: Adherent
- Model: Tumour line
- Conditional: Yes
- Description: Established from a squamous cell carcinoma (SCC) of the buccal mucosa (20mm-40mm) of a male patient, age 84. STNMP stage II, well differentiated, node positive tumour. Mutant p53, codon 306 exon 8, G to A; wild type K-, N- and Ha-ras. Non-tumourigenic in athymic nude mice by subcutaneous injection and on injection into the floor of the mouth (orthotopic), but form epidermoid cysts subcutaneously
- Cellosaurus id: CVCL_2458
Target Details
- Target: Human oral squamous cell carcinoma
Handling
- Format: Frozen
- Growth medium: Split sub-confluent cultures (70-80%) 1:8 to 1:10 5% CO2; 37?°C. Suggested seeding density 5 x 1000 cells/cm?Â. DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.
- Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.
- Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.
- Epidermal growth factor and transforming growth factor alpha characteristics of human oral carcinoma cell lines.
- Yeudall et al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-7. PMID: 8180579.
- Ras gene point mutation is a rare event in premalign...