Cat. #153190
Anti-Phospho-Rb (Thr356) [7F10]
Cat. #: 153190
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 10-12 weeks
Target: Rb (phospho Thr356)
Class: Monoclonal
Application: IHC ; WB
Reactivity: Human ; Mouse
Host: Rat
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Sibylle Mittnacht
Institute: The Institute of Cancer Research
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-Phospho-Rb (Thr356) [7F10]
- Alternate name: Retinoblastoma 1; RB1; p15-Rb; RB; pp11; pRb; OSRC
- Clone: 7F10
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 111 kDa
- Reactivity: Human ; Mouse
- Host: Rat
- Application: IHC ; WB
- Description: Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia. Phosphorylation of Rb at S608 depends on CDK4.
- Immunogen: Synthetic peptide COOH-C-ERERpTPRKNN-NH2 coupled to mycobacterium tuberculosis PPD
- Isotype: IgG2a
Target Details
- Target: Rb (phospho Thr356)
- Molecular weight: 111 kDa
- Target background: Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia. Phosphorylation of Rb at S608 depends on CDK4.
Applications
- Application: IHC ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Barrie et al. 2003. Anal Biochem. 320(1):66-74. PMID: 12895470.
- High-throughput screening for the identification of small-molecule inhibitors of retinoblastoma protein phosphorylation in cells.
