Cat. #152728
Anti-P15PAF [K11-P1G7*B8]
Cat. #: 152728
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: PCNA-Associated Factor of 15 (PAF15)
Class: Monoclonal
Application: ELISA ; IHC ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Ayham Alnabulsi
Institute: Vertebrate Antibodies Limited
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-P15PAF [K11-P1G7*B8]
- Clone: K11-P1G7*B8
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; IHC ; WB
- Description: Proliferating cell nuclear antigen (PCNA)-Associated Factor (PAF15) is a small protein containing a PCNA interacting motif and sequences for association with ubiquitin enzymes. In interaction with PCNA, PAF15 plays a key role in recruiting DNA replicative polymerase by double monoubiquitination at Lys(15) and Lys(24). Under DNA damage conditions, PAF15 regulates the switch from DNA replicative polymerase to translesion synthesis polymerase in order to bypass the replication-blocking lesions. Overexpression of PAF15 promotes the repair of ultraviolet-induced DNA damage and prevents cell death, whereas attenuation of PAF15 decreases DNA replication and cell survival.
- Immunogen: Peptide Sequence RKACPLQPDHT -peptide immunogen is identical in mouse (Mus musculus) and rat (Rattus norvegicus).
- Isotype: IgG2b kappa
- Myeloma used: P3X63Ag8.653
- Recommended controls: ELISA - peptide immunogen; WB - Jurkat whole cell lysate; IHC - formalin-fixed, paraffin-embedded multi tumour tissue microarray
Target Details
- Target: PCNA-Associated Factor of 15 (PAF15)
- Tissue cell line specificity: ELISA - peptide immunogen; WB - Jurkat whole cell lysate; IHC - formalin-fixed, paraffin-embedded multi tumour tissue microarray
- Target background: Proliferating cell nuclear antigen (PCNA)-Associated Factor (PAF15) is a small protein containing a PCNA interacting motif and sequences for association with ubiquitin enzymes. In interaction with PCNA, PAF15 plays a key role in recruiting DNA replicative polymerase by double monoubiquitination at Lys(15) and Lys(24). Under DNA damage conditions, PAF15 regulates the switch from DNA replicative polymerase to translesion synthesis polymerase in order to bypass the replication-blocking lesions. Overexpression of PAF15 promotes the repair of ultraviolet-induced DNA damage and prevents cell death, whereas attenuation of PAF15 decreases DNA replication and cell survival.
Applications
- Application: ELISA ; IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
