Cat. #152706
A2780 Cell Line
Cat. #: 152706
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Ovary
Disease: Cancer
Model: Tumour line
£800.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: National Cancer Institute
Tool Details
*FOR RESEARCH USE ONLY
- Name: A2780 Cell Line
- Alternate name: A-2780 Cells; A2780 ovarian cancer cell line
- Organism: Human
- Tissue: Ovary
- Disease: Cancer
- Growth properties: Adherent
- Model: Tumour line
- Conditional: Yes
- Description: Access A2780 the gold standard human ovarian cancer cell line. A2780 cell line was established from an ovarian endometroid adenocarcinoma tumour tissue of an untreated patient. It is widely used as a model to observe the effects of treatment delivery and toxicity. This epithelial cell line grows as a monolayer, in suspension in spinner cultures, and is tumourigenic in immune deficient mice. A2780 displays cisplatin-sensitivity. Our collection also features seven A2780 derivatives, including two drug resistant cell line variants: a cisplatin resistant cell line - A2780 cis (catalogue no. 152708) and the adriamycin resistant cell line - A2780 ADR (catalogue no. 152707). Published uses of this line include: (1) Investigating the role of ovarian tumour neovascularisation (Abdulkhalek et al. 2014). (2) Resistance to chemotherapeutic drugs. (Pfankuchen et al. 2015). (3) Demonstrating significance of therapeutic targets in ovarian cancer
- Application: A2780 cell line is used in toxicity testing and cancer genetic studies.
- Production details: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 3-6x10,000cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
- Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 12,13 D16S539: 11,13 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,10 vWA: 15,16
- Cellosaurus id: CVCL_0134
Applications
- Application: A2780 cell line is used in toxicity testing and cancer genetic studies.
- Application notes: Points of Interest STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 12,13 D16S539: 11,13 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,10 vWA: 15,16 Concentration Vial has between 1-5 million cells as standard, however this may vary.
Handling
- Format: Frozen
- Growth medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
Related Tools
- Related tools: A2780ADR Cell Line ; A2780cis Cell Line
References
- Nusinow et al. 2020. Cell. 180(2):387-402.e16. PMID: 31978347.
- Ghandi et al. 2019. Nature. 569(7757):503-508. PMID: 31068700.
- Dutil et al. 2019. Cancer Res. 79(7):1263-1273. PMID: 30894373.
- Zhao et al. 2017. Clin Proteomics. 14:20. PMID: 28546799.
- Medrano et al. 2017. Cell Rep. 18(10):2343-2358. PMID: 28273451.
- Pfankuchen et al. 2015. Biochem Pharmacol. 97(2):147-57. PMID: 26239805.
- Abdulkhalek et al. 2014. Clin Transl Med. 3(1):28. PMID: 26932374.
- Januchowski et al. 2014. Biomed Res Int. 2014:365867. PMID: 24804215
- Parker et al. 1991. J Clin Invest. 87(3):772-777. PMID: 1999494.
