A2780 Cell Line

Tool Details

*FOR RESEARCH USE ONLY

• Name: A2780 Cell Line
• Alternate name: A-2780 Cells; A2780 ovarian cancer cell line
• Organism: Human
• Tissue: Ovary
• Disease: Cancer
• Growth properties: Adherent
• Model: Tumour line
• Conditional: Yes
• Description: Access A2780 the gold standard ovarian cancer cell line. A2780 cell line was established from an ovarian endometroid adenocarcinoma tumour tissue of an untreated patient. It is widely used as a model to observe the effects of treatment delivery and toxicity. This epithelial line grows as a monolayer, in suspension in spinner cultures, and is tumourigenic in immune deficient mice. A2780 displays cisplatin-sensitivity. Our collection also features seven A2780 derivatives, including two drug resistant cell line variants: a cisplatin resistant cell line – A2780 cis (catalogue no. 152708) and the adriamycin resistant cell line - A2780 ADR (catalogue no. 152707). Published uses of this line include: (1) Investigating the role of ovarian tumour neovascularisation (Abdulkhalek et al. 2014). (2) Resistance to chemotherapeutic drugs (Pfankuchen et al. 2015). (3) Demonstrating significance of therapeutic targets in ovarian cancer.
• Application: A2780 cell line is used in toxicity testing and cancer genetic studies.
• Production details: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 3-6x10,000cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
• Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 12,13 D16S539: 11,13 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,10 vWA: 15,16
• Cellosaurus id: CVCL_0134

Applications

• Application: A2780 cell line is used in toxicity testing and cancer genetic studies.
• Application notes: Points of Interest STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 12,13 D16S539: 11,13 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,10 vWA: 15,16 Concentration Vial has between 1-5 million cells as standard, however this may vary.

Handling

• Format: Frozen
• Growth medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice

Related Tools

• Related tools: A2780ADR Cell Line ; A2780cis Cell Line

References

• Nusinow et al. 2020. Cell. 180(2):387-402.e16. PMID: 31978347.
• Ghandi et al. 2019. Nature. 569(7757):503-508. PMID: 31068700.
• Dutil et al. 2019. Cancer Res. 79(7):1263-1273. PMID: 30894373.
• Zhao et al. 2017. Clin Proteomics. 14:20. PMID: 28546799.
• Medrano et al. 2017. Cell Rep. 18(10):2343-2358. PMID: 28273451.
• Pfankuchen et al. 2015. Biochem Pharmacol. 97(2):147-57. PMID: 26239805.
• Abdulkhalek et al. 2014. Clin Transl Med. 3(1):28. PMID: 26932374.
• Januchowski et al. 2014. Biomed Res Int. 2014:365867. PMID: 24804215
• Parker et al. 1991. J Clin Invest. 87(3):772-777. PMID: 1999494.