#152558

MCF7/ExeR-4 Cell Line

Cat. #152558

MCF7/ExeR-4 Cell Line

Cat. #: 152558

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 10-12 weeks

Organism: Human

Tissue: Breast

Disease: Cancer

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Anne Lykkesfeldt

Institute: Danish Cancer Society, Denmark

Primary Citation: Hole et al. 2015. Int J Oncol. 46(4):1481-1490. PMID: 25625755.

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: MCF7/ExeR-4 Cell Line
  • Alternate name: MCF-7/ExeR-4; ExeR-4
  • Tool sub type: Continuous
  • Parental cell: MCF7
  • Organism: Human
  • Gender: Female
  • Tissue: Breast
  • Disease: Cancer
  • Growth properties: Breast cancer cell line resistant to the aromatase inhibitor exemestane. Estrogen receptor positive.
  • Conditional: No
  • Description: MCF7/ExeR-4 is a cell culture model mimicking acquired resistance of aromatase inhibitors (AIs) - an anti-cancer therapy. This breast cancer cell line was established from MCF7 cells. The cellular classification is epithelial, and their shape is polygonal.

    The MCF7/ExeR-4 cell line is resistant to the third generation AI - Exemestane (Aromasin). Third generation AIs have proven to be effective treatment for estrogen receptor positive (ER+) breast cancer and as such are recommended a...
  • Application: Investigation of molecular mechanisms of exemestane resistance
  • Production details: Exemestane-resistant cell lines were established from MCF-7 cells grown in medium with 10% NCS and 10-7 M testosterone. A culture of MCF-7 cells were treated with 10-7 M exemetane for one week, trypsinised and seeded in serial dilutions in 24-well plates. Single colonies were transferred to new wells and gradually expanded in medium with exemestane. After ~2-3 months, the isolated colonies gave rise to exemestane-resistant cell lines, which could be grown in exemestane-containing medium with ...
  • Cellosaurus id: CVCL_5A16

Target Details

  • Target: Exemestane resistance

Applications

  • Application: Investigation of molecular mechanisms of exemestane resistance
  • Application notes: Human breast cancer cell line derived from MCF-7 cells Other related cell lines: - LetR-1, LetR-2, LetR-3 and LetR-4 resistant to the non-steroidal AI letrozole - ExeR-1, ExeR-2 and ExeR-3 resistant to the steroidal AI exemestane - AnaR-1, AnaR-2, AnaR-3 and AnaR-4 resistant to the non-steroidal AI anastrozole Passage 432 (AL3949, AL3950)

Handling

  • Format: Frozen
  • Passage number: Passage 432 (AL3949, AL3950)
  • Growth medium: Phenol-red-free DMEM/F12 medium supplemented with 10% newborn calf serum, 2.5 mM Glutamax, 6 ng/ ml insulin, 0.1 uM testosterone and 0.1 uM exemestane.
  • Temperature: 37° C
  • Atmosphere: Humidified air with 5% CO2
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

Related Tools

  • Related tools: MCF7/ExeR-1 Cell Line Other related cell lines: - LetR-1, LetR-2, LetR-3 and LetR-4 resistant to the non-steroidal AI letrozole - ExeR-1, ExeR-2 and ExeR-3 resistant to the steroidal AI exemestane - AnaR-1, AnaR-2, AnaR-3 and AnaR-4 resistant to the non-steroidal AI anastrozole

References

  • Hole et al. 2015. Breast Cancer Res Treat. 149(3):715-726. PMID: 25667100.
  • Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755.