Cat. #152558
MCF7/ExeR-4 Cell Line
Cat. #: 152558
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Human
Tissue: Breast
Disease: Cancer
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Anne Lykkesfeldt
Institute: Danish Cancer Society, Denmark
Primary Citation: Hole et al. 2015. Int J Oncol. 46(4):1481-1490. PMID: 25625755.
Tool Details
*FOR RESEARCH USE ONLY
- Name: MCF7/ExeR-4 Cell Line
- Alternate name: MCF-7/ExeR-4; ExeR-4
- Tool sub type: Continuous
- Parental cell: MCF7
- Organism: Human
- Gender: Female
- Tissue: Breast
- Disease: Cancer
- Growth properties: Breast cancer cell line resistant to the aromatase inhibitor exemestane. Estrogen receptor positive.
- Conditional: No
- Description: MCF7/ExeR-4 is a cell culture model mimicking acquired resistance of aromatase inhibitors (AIs) - an anti-cancer therapy. This breast cancer cell line was established from MCF7 cells. The cellular classification is epithelial, and their shape is polygonal.
The MCF7/ExeR-4 cell line is resistant to the third generation AI - Exemestane (Aromasin). Third generation AIs have proven to be effective treatment for estrogen receptor positive (ER+) breast cancer and as such are recommended a... - Application: Investigation of molecular mechanisms of exemestane resistance
- Production details: Exemestane-resistant cell lines were established from MCF-7 cells grown in medium with 10% NCS and 10-7 M testosterone. A culture of MCF-7 cells were treated with 10-7 M exemetane for one week, trypsinised and seeded in serial dilutions in 24-well plates. Single colonies were transferred to new wells and gradually expanded in medium with exemestane. After ~2-3 months, the isolated colonies gave rise to exemestane-resistant cell lines, which could be grown in exemestane-containing medium with ...
- Cellosaurus id: CVCL_5A16
Target Details
- Target: Exemestane resistance
Applications
- Application: Investigation of molecular mechanisms of exemestane resistance
- Application notes: Human breast cancer cell line derived from MCF-7 cells Other related cell lines: - LetR-1, LetR-2, LetR-3 and LetR-4 resistant to the non-steroidal AI letrozole - ExeR-1, ExeR-2 and ExeR-3 resistant to the steroidal AI exemestane - AnaR-1, AnaR-2, AnaR-3 and AnaR-4 resistant to the non-steroidal AI anastrozole Passage 432 (AL3949, AL3950)
Handling
- Format: Frozen
- Passage number: Passage 432 (AL3949, AL3950)
- Growth medium: Phenol-red-free DMEM/F12 medium supplemented with 10% newborn calf serum, 2.5 mM Glutamax, 6 ng/ ml insulin, 0.1 uM testosterone and 0.1 uM exemestane.
- Temperature: 37° C
- Atmosphere: Humidified air with 5% CO2
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
Related Tools
- Related tools: MCF7/ExeR-1 Cell Line Other related cell lines: - LetR-1, LetR-2, LetR-3 and LetR-4 resistant to the non-steroidal AI letrozole - ExeR-1, ExeR-2 and ExeR-3 resistant to the steroidal AI exemestane - AnaR-1, AnaR-2, AnaR-3 and AnaR-4 resistant to the non-steroidal AI anastrozole
References
- Hole et al. 2015. Breast Cancer Res Treat. 149(3):715-726. PMID: 25667100.
- Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755.