#152108

T47D/TR-1 Cell Line

Cat. #152108

T47D/TR-1 Cell Line

Cat. #: 152108

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-5 days

Organism: Human

Tissue: Breast

Disease: Cancer

Model: Tumour line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Anne Lykkesfeldt

Institute: Danish Cancer Society

Primary Citation: Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: T47D/TR-1 Cell Line
  • Tool sub type: Continuous
  • Parental cell: T47D/S2
  • Organism: Human
  • Tissue: Breast
  • Disease: Cancer
  • Model: Tumour line
  • Conditional: Yes
  • Description: The T47D/TR-1 cell line is a breast cancer cell line resistant to fulvestrant (Faslodex). This cell line has been established from T47D/S2 cells by long term treatment with 1 nM tamoxifen. T47D/TR-1 is adherent and the morphology is polygonal epithelial. T47D/TR-1 cells are oestrogen receptor (alpha) positive, they also express progesterone receptor. T47D/TR-1 are growth inhibited by fulvestrant. Antioestrogen resistance is a major problem in breast cancer treatment. Ther...
  • Application: Determining molecular mechanisms around tamoxifen resistance
  • Production details: The T47D/TR-1 cell line has been established by long term treatment of T47D/S2 cells with 1 uM tamoxifen. Clonal selection was performed in medium without tamoxifen. After growth in presence of 1 uM tamoxifen for 10 months, the growth rate was similar to the parental cells.
  • Biosafety level: 1
  • Cellosaurus id: CVCL_1D36

Target Details

  • Target: Oestrogen receptor

Applications

  • Application: Determining molecular mechanisms around tamoxifen resistance
  • Application notes: T47D/TR-1 cells are ER alpha positive and express progesterone receptor, although at reduced level compared to parental T7D/S2 cells. T47D/TR-1 are growth inhibited by fulvestrant.

Handling

  • Format: Frozen
  • Passage number: Passage 164 (AL3569, AL3570)
  • Growth medium: Phenol red free RPMI 1640 + 2% FCS + glutamax + 8ug Insulin/ml + 1 uM tamoxifen
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: T47D/S2 Cell Line

References

  • Larsen et al. 2015. PLoS One. 10(2):e0118346. PMID: 25706943.
  • Larsen et al. 2015. BMC Cancer. 15(1):1-15. PMID: 25885472.
  • Thrane et al. 2014. Oncogene. 34(32):4199-4210. PMID: 25362855.
  • Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.