Cat. #152108
T47D/TR-1 Cell Line
Cat. #: 152108
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 3-5 days
Organism: Human
Tissue: Breast
Disease: Cancer
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Anne Lykkesfeldt
Institute: Danish Cancer Society
Primary Citation: Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.
Tool Details
*FOR RESEARCH USE ONLY
- Name: T47D/TR-1 Cell Line
- Tool sub type: Continuous
- Parental cell: T47D/S2
- Organism: Human
- Tissue: Breast
- Disease: Cancer
- Model: Tumour line
- Conditional: Yes
- Description: The T47D/TR-1 cell line is a breast cancer cell line resistant to fulvestrant (Faslodex). This cell line has been established from T47D/S2 cells by long term treatment with 1 nM tamoxifen. T47D/TR-1 is adherent and the morphology is polygonal epithelial. T47D/TR-1 cells are oestrogen receptor (alpha) positive, they also express progesterone receptor. T47D/TR-1 are growth inhibited by fulvestrant. Antioestrogen resistance is a major problem in breast cancer treatment. Ther...
- Application: Determining molecular mechanisms around tamoxifen resistance
- Production details: The T47D/TR-1 cell line has been established by long term treatment of T47D/S2 cells with 1 uM tamoxifen. Clonal selection was performed in medium without tamoxifen. After growth in presence of 1 uM tamoxifen for 10 months, the growth rate was similar to the parental cells.
- Biosafety level: 1
- Cellosaurus id: CVCL_1D36
Target Details
- Target: Oestrogen receptor
Applications
- Application: Determining molecular mechanisms around tamoxifen resistance
- Application notes: T47D/TR-1 cells are ER alpha positive and express progesterone receptor, although at reduced level compared to parental T7D/S2 cells. T47D/TR-1 are growth inhibited by fulvestrant.
Handling
- Format: Frozen
- Passage number: Passage 164 (AL3569, AL3570)
- Growth medium: Phenol red free RPMI 1640 + 2% FCS + glutamax + 8ug Insulin/ml + 1 uM tamoxifen
- Temperature: 37° C
- Atmosphere: 5% CO2
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
Related Tools
- Related tools: T47D/S2 Cell Line
References
- Larsen et al. 2015. PLoS One. 10(2):e0118346. PMID: 25706943.
- Larsen et al. 2015. BMC Cancer. 15(1):1-15. PMID: 25885472.
- Thrane et al. 2014. Oncogene. 34(32):4199-4210. PMID: 25362855.
- Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.