Anti-APC [c-APC 28.9]

Tool Details

*FOR RESEARCH USE ONLY

• Name: Anti-APC [c-APC 28.9]
• Clone: c-APC 28.9
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 320 kDa
• Reactivity: Human
• Host: Mouse
• Application: IHC ; IF ; IP ; WB
• Description: C-APC 28.8 can be used for APC expression and detection of APC mutations.
• Immunogen: human APC C-terminal fusion with MBP (maltose binding protein)
• Isotype: IgG1 kappa
• Recommended controls: Colon cell line HCT116

Target Details

• Target: APC
• Molecular weight: 320 kDa
• Tissue cell line specificity: Colon cell line HCT116
• Target background: The adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. Clone c-APC 28.9 is useful for the detection of APC expression and detection of mutations. This antibody has been used routinely for Western blotting on colon cell line HCT116 extract which expresses full-length APC.

Applications

• Application: IHC ; IF ; IP ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Shipping at 4° C

References

• Kariola et al. 2005. Fam Cancer. 4(2):187-90. PMID: 15951972.
• APC and beta-catenin protein expression patterns in HNPCC-related endometrial and colorectal cancers.
• Fodde et al. 2001. Nat Cell Biol. 3(4):433-8. PMID: 11283620.
• Mutations in the APC tumour suppressor gene cause chromosomal instability.