MCF7/TAMR-7 Cell line

Tool Details

*FOR RESEARCH USE ONLY

• Name: MCF7/TAMR-7 Cell line
• Alternate name: MCF-7/TAMR-7; TAMR-7
• Tool sub type: Continuous
• Parental cell: MCF7 S0.5
• Organism: Human
• Gender: Female
• Tissue: Breast
• Disease: Cancer
• Model: Tumour line
• Conditional: Yes
• Description: MCF7/TAMR-7 cell line is a stable tamoxifen-resistant subline. The cell line has been established in tissue culture after long term treatment with 1uM tamoxifen. Tamoxifen (Nolvadex) is a widely used drug for hormone-dependent cancer. Tamoxifen resistance (either primary or acquired) makes oestrogen receptor-positive breast cancer much more difficult to treat. This cell line was produced as an adaptation of an original cell line (MCF7/S0.5), as a model cell system to study the effects of tamoxifen resistant cancer growth. MCF7/TAMR-7 cells are oestrogen receptor positive and progesterone receptor negative. Previous applications of this cell line include treatment with steroidal antiestrogens. MCF7/TAMR-7 enables identification of new hormone therapies and greater understanding of the signalling pathways/methods behind tamoxifen resistance.
• Application: Investigate signalling pathways involved in tamoxifen resistance
• Production details: The parental cell line for the MCF7/TAMR-7 cells is MCF7/S0.5, which was adapted to grow at low serum concentration in order to study the effect of estradiol and tamoxifen. MCF7/TAMR-7 has been established from a clone of cells that survived long term treatment with 1 uM tamoxifen. Tamoxifen-resistant cells are passaged continuously in presence of 1 uM tamoxifen, which is lethal for the parental MCF7/S0.5 cell line.
• Recommended controls: MCF7-S0.5 parental line
• Cellosaurus id: 1D43

Target Details

• Target: Oestrogen receptor

Applications

• Application: Investigate signalling pathways involved in tamoxifen resistance
• Application notes: MCF7/TAMR-7 cells are oestrogen receptor positive and progesterone receptor negative. MCF7/TAMR-7 cells are growth inhibited by the pure antioestrogen fulvestrant. The oestrogen receptor is a major driver of growth of MCF7/TAMR-7 cell. The TAMR lines were established from the MCF7/S0.5 cell line, which was adapted to grow with 0.5% fetal calf serum in phenol red containing DMEM/F12 medium. Treatment with tamoxifen was started in passage 351. Few colonies of cells survived the treatment and a...

Handling

• Format: Frozen
• Growth medium: Phenol red-free DMEM:Ham's F-12 containing 1% fetal bovine serum, 2 mM Glutamax and 6 ng/ml insulin. To maintain high-level resistance, medium was supplemented with Tamoxifen (1 uM).
• Temperature: 37° C
• Atmosphere: 5% CO2
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice

Related Tools

• Related tools: MCF7/S0.5 Cell Line

References

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• Lundqvist et al. 2014. Steroids. 85:30-35. PMID: 24747771.
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• Berstein e...