#151741

Anti-Keratin [LDS103]

Cat. #151741

Anti-Keratin [LDS103]

Cat. #: 151741

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 10-12 weeks

Target: Keratin expressed in basal cells, pan-epithelial

Class: Monoclonal

Application: IHC ; IF

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Jenny Southgate

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-Keratin [LDS103]
  • Clone: LDS103
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; IF
  • Description: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 17 and 18 are type I cytokeratins. Keratin 17 is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It has been shown to interact with CCDC85B. Keratin 18 is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. Keratin 18 has been shown to interact with Collagen, type XVII, alpha 1, DNAJB6, Pinin and TRADD.
  • Immunogen: Cytoskeletons of the human urothelial RT4 cell line.
  • Isotype: IgG1

Target Details

  • Target: Keratin expressed in basal cells, pan-epithelial
  • Target background: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 17 and 18 are type I cytokeratins. Keratin 17 is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It has been shown to interact with CCDC85B. Keratin 18 is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. Keratin 18 has been shown to interact with Collagen, type XVII, alpha 1, DNAJB6, Pinin and TRADD.

Applications

  • Application: IHC ; IF

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Trejdosiewicz et al. 1986. Exp Cell Res. 164(2):388-98. PMID: 2423348.
  • Phenotypic analysis of cultured melanoma cells. Expression of cytokeratin-type intermediate filaments by the M5 human melanoma cell line.