Cat. #151682
K38 Keratinocyte Cell Line
Cat. #: 151682
Unit size: 1x10^6 cells / vial
Organism: Mouse
Disease: Dermatology
Model: Primary line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Julia Reichelt
Institute: Newcastle University
Tool Details
*FOR RESEARCH USE ONLY
- Name: K38 Keratinocyte Cell Line
- Organism: Mouse
- Disease: Dermatology
- Growth properties: Adherent
- Model: Primary line
- Description: The K38 Keratinocyte Cell Line has been established from the skin of neonatal WT mice. The prospective commercial application of the line is in the generation of in vitro epidermal 'skin equivalents' to be used as screening tools to look at the activity of NCE's and/or NBE's of dermatological interest, and/or to screen for such NCE and/or NBE in high throughput systems. It may also be of use to non-pharmaceutical healthcare and/or cosmetic companies involved in the personal vitality and/or beauty product development segments Murine keratinocyte stem cell line
- Production details: Keratinocytes were isolated from neonatal wild-type BALB/c mouse skin. The cell line was established by growing cells for the first 8 passages in co-culture with 3T3-J2 fibroblast feeder cells. From passage 9 onwards cells were grown without feeder cells.
- Cellosaurus id: CVCL_W141
Handling
- Format: Frozen
- Growth medium: The cell line grown is low calcium medium, Ham's F12/DMEM with 10% FCS and several supplements at 32?°C, 5% CO2. The supplements are: 0.18 mM adenine, 0.5 ??g/ml hydrocortisone, 5 ??g/ml insulin,10 -10 M cholera toxin, 10 ng/ml EGF, 2 mM glutamine, 1 mM
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Reichelt and Haase, 2010. Methods Mol Biol. 585:59-69. PMID: 19907996 HÄ?her T et al., 2011. Stem Cell Rev. PMID: 21874280 Vollmers A et al., 2011. PMID: 21892602
