#151651

Anti-WIPI2 [2A2]

Cat. #151651

Anti-WIPI2 [2A2]

Cat. #: 151651

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: WIPI2

Class: Monoclonal

Application: IF ; FACS ; IF ; IP ; WB

Reactivity: Human ; Mouse

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Sharon Tooze

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-WIPI2 [2A2]
  • Clone: 2A2
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 49 kDa
  • Reactivity: Human ; Mouse
  • Host: Mouse
  • Application: IF ; FACS ; IF ; IP ; WB
  • Description: This antibody is useful for the detection of WIPI-2, an essential protein for autophagy.
  • Immunogen: Synthetic peptide corresponding to C-terminus of WIPI2b CSALRLDEDSEHPPMILRTD
  • Isotype: IgG1 kappa
  • Recommended controls: HEK293 cells

Target Details

  • Target: WIPI2
  • Molecular weight: 49 kDa
  • Tissue cell line specificity: HEK293 cells
  • Target background: WIPI2 is a mammalian effector of PtdIns3P and is ubiquitously expressed in a variety of cell lines. WIPI2 is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. WIPI-2 is the mammalian homologue of Atg18.

Applications

  • Application: IF ; FACS ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Nishimura et al. 2017. EMBO J. 36(12):1719-1735. PMID: 28495679.
  • Autophagosome formation is initiated at phosphatidylinositol synthase-enriched ER subdomains.
  • Martinez-Martin et al. 2017. Science. 355(6325):641-647. PMID: 28183981.
  • A switch from canonical to noncanonical autophagy shapes B cell responses.
  • Polson et al. 2010. Autophagy. 6(4):506-22. PMID: 20505359.
  • Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.
  • Li et al. 2005. J Biol Chem. 280(29):26941-52. PMID: 15917248.
  • Krppel-like factor-6 promotes preadipocyte differentiation through histone deacetylase 3-dependent repression of DLK1.
  • Benzeno et al. 2004. Cancer Res. 64(11):3885-91. PMID: 15172998.
  • Cyclin-dependent kinase inhibition by the KLF6 tumor suppressor protein through interaction with cyclin D1.