Cat. #151558
MEF Trex1 KO Cell Line
Cat. #: 151558
Unit size: 1x10^6 cells / vial
Organism: Mouse
Tissue: Embryo
Disease: Trex1-deficient Aicardi-Goutieres syndrome
Model: Knock-Out
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Deborah Barnes
Institute: Cancer Research UK, London Research Insitute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY
- Name: MEF Trex1 KO Cell Line
- Organism: Mouse
- Tissue: Embryo
- Disease: Trex1-deficient Aicardi-Goutieres syndrome
- Growth properties: Defective G1/S transition, increased cell doubling time; chronic ATM-dependent checkpoint activation even in the absence of exogenous stress; persistant ssDNA polynucleotide species generated in S phase that accumulates in the cytoplasm.
- Model: Knock-Out
- Conditional: Yes
- Description: The Trex1 KO MEF cell line is a spontaneously transformed mouse embryonic fibroblast (MEF) cell line. It allows the study of novel interconnections between DNA replication, DNA damage checkpoint signalling and antiviral-like autoimmune responses. Recapitulates cellular phenotype of Trex1-deficient Aicardi-Goutieres syndrome. Spontaneously transformed clone derived following repeated passaging in culture by standard techniques of primary MEFs from Trex1-/- knockout mouse
- Production details: MEF from Trex1-/- knockout mouse
- Cellosaurus id: CVCL_5A06
Target Details
- Target: TREX1
Applications
- Application notes: Spontaneously transformed clone derived following repeated passaging in culture by standard techniques of primary MEFs from Trex1-/- knockout mouse
Handling
- Format: Frozen
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Vapor phase of liquid nitrogen. Storage at -70° C will result in loss of viability.
Related Tools
- Related tools: Trex1 -/- Mouse
References
- Yang et al. 2007. Cell. 131(5):873-86. PMID: 18045533.
- Trex1 exonuclease degrades ssDNA to prevent chronic checkpoint activation and autoimmune disease.
- Morita et al. 2004. Mol Cell Biol. 24(15):6719-27. PMID: 15254239.
- Gene-targeted mice lacking the Trex1 (DNase III) 3'-->5' DNA exonuclease develop inflammatory myocarditis.
