#151528

Anti-von Willebrand Factor [RFF-VIII:R/1]

Cat. #151528

Anti-von Willebrand Factor [RFF-VIII:R/1]

Cat. #: 151528

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 10-12 weeks

Target: Von Willebrand factor (VWF)

Class: Monoclonal

Application: ELISA ; FACS ; IF ; Fn ; RIA ; WB

Reactivity: Primate

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Alison Goodall

Institute: University College London (UCL)

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-von Willebrand Factor [RFF-VIII:R/1]
  • Clone: RFF-VIII:R/1
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Purpose: Marker
  • Conjugation: Unconjugated
  • Reactivity: Primate
  • Host: Mouse
  • Application: ELISA ; FACS ; IF ; Fn ; RIA ; WB
  • Description: Anti-Anti-Von Willebrand Factor [RFF-VIII:R/1] specifically binds to human factor VIII related antigen (VIII:RAg) in plasma and in vascular endothelial cells but has no reactivity with factor VIII procoagulant antigen (VIII:cAg). This antibody is a potent inhibitor of von Willebrand factor activity in that it can totally neutralise risocetin-induced aggregation of platelet rich plasma and inhibit platelet adhesion at high flow rates. RFF-VIII:R/2 can be used in a one-stage, fluid phase immuno...
  • Immunogen: Purified human Factor VIII-VWF complex
  • Isotype: IgG1 kappa
  • Myeloma used: P3/NS1/1-Ag4.1

Target Details

  • Target: Von Willebrand factor (VWF)
  • Tissue cell line specificity: Mouse
  • Target background: Anti-Anti-Von Willebrand Factor [RFF-VIII:R/1] specifically binds to human factor VIII related antigen (VIII:RAg) in plasma and in vascular endothelial cells but has no reactivity with factor VIII procoagulant antigen (VIII:cAg). This antibody is a potent inhibitor of von Willebrand factor activity in that it can totally neutralise risocetin-induced aggregation of platelet rich plasma and inhibit platelet adhesion at high flow rates. RFF-VIII:R/2 can be used in a one-stage, fluid phase immuno...

Applications

  • Application: ELISA ; FACS ; IF ; Fn ; RIA ; WB

Handling

  • Format: Liquid
  • Unit size: 100 ug
  • Shipping conditions: Shipping at 4° C

References

  • [Use of RFF-VIII:R/1 in the purification of FVIII]
  • Murdock et al. 1997. Thromb Haemost. 78(4):1272-7. PMID: 9364997.
  • von Willebrand factor activity detected in a monoclonal antibody-based ELISA: an alternative to the ristocetin cofactor platelet agglutination assay for diagnostic use.
  • Takase et al. 1987. Br J Haematol. 66(4):497-502. PMID: 3117089.
  • Production of factor VIII deficient plasma by immunodepletion using three monoclonal antibodies.
  • McCraw et al. 1987. Thromb Res. 45(1):1...