#151510

Anti-Zebrafish DeltaC [zdc2]

Cat. #151510

Anti-Zebrafish DeltaC [zdc2]

Cat. #: 151510

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Zebrafish DeltaC

Class: Monoclonal

Application: ELISA ; FACS ; IHC ; IF ; WB

Reactivity: Zebrafish

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Gavin Wright

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-Zebrafish DeltaC [zdc2]
  • Clone: zdc2
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Strain: Balb/c
  • Reactivity: Zebrafish
  • Host: Mouse
  • Application: ELISA ; FACS ; IHC ; IF ; WB
  • Description: Delta proteins activate Notch through a binding reaction that depends on their extracellular domains. During somitogenesis, Notch signalling via DeltaC keeps the oscillations of neighbouring cells in synchrony. DeltaC is also expressed in subsets of cells in the nervous system and elsewhere.
  • Immunogen: Extracellular region of zebrafish DeltaC protein
  • Isotype: IgG2a
  • Myeloma used: Sp2/0-Ag14
  • Recommended controls: HEK293 cells transfected with DeltaD and Zebrafish embryo cryosections (formulin fixed)

Target Details

  • Target: Zebrafish DeltaC
  • Tissue cell line specificity: HEK293 cells transfected with DeltaD and Zebrafish embryo cryosections (formulin fixed)
  • Target background: Delta proteins activate Notch through a binding reaction that depends on their extracellular domains. During somitogenesis, Notch signalling via DeltaC keeps the oscillations of neighbouring cells in synchrony. DeltaC is also expressed in subsets of cells in the nervous system and elsewhere.

Applications

  • Application: ELISA ; FACS ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -80° C
  • Shipping conditions: Shipping at 4° C

References

  • Gallagher et al. 2017. Dev Biol. 429(1):225-239. PMID: 28648842.
  • Quillien et al. 2014. Development. 141(7):1544-52. PMID: 24598161.
  • Wright et al. 2011. Development. 138(14):2947-56. PMID: 21653612.
  • DeltaC and DeltaD interact as Notch ligands in the zebrafish segmentation clock.
  • Giudicelli et al. 2007. PLoS Biol. 5(6):e150. PMID: 17535112.
  • Setting the tempo in development: an investigation of the zebrafish somite clock mechanism.