Cat. #151482
Jagged1 Floxed Mouse
Cat. #: 151482
Sub-type: Mouse
Availability: 6-8 weeks
Disease: Cancer; Autoimmune disease
Model: Knock-Out
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Contributor
Inventor: Julian Lewis
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY
- Tool name: Jagged1 Floxed Mouse
- Tool sub type: Mouse
- Disease: Cancer; Autoimmune disease
- Model: Knock-Out
- Conditional: Yes
- Conditional description: Jag1 gene with loxP sites flanking exons 4 & 5 enabling conditional Cre-mediate knockout.
- Description: Conditional knockout of Jag1; in vivo studies of tissue-specific Jag1 knockout and Notch signalling
- Genetic background: A Jag1 targeting vector, containing a loxP flanked resistance cassette before exon 4 and a loxP site inserted after exon 5, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were transfected with a Cre expression vector to excise the resistance cassette, and injected into a C57BL6 blastocyst. Chimeric offspring were mated on a C57BL6 background.
- Zygosity: Heterozygous
- Production details: A Jag1 targeting vector, containing a loxP flanked resistance cassette before exon 4 and a loxP site inserted after exon 5, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were transfected with a Cre expression vector to excise the resistance cassette, and injected into a C57BL6 blastocyst. Chimeric offspring were mated on a C57BL6 background.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Jagged1 (Jag1)
References
- Brooker et al. 2006. Development. 133(7):1277-86. PMID: 16495313.
- Notch ligands with contrasting functions: Jagged1 and Delta1 in the mouse inner ear.
