Cat. #151476
Raf KO Mouse
Cat. #: 151476
Sub-type: Mouse
Availability: 6-8 weeks
Disease: Cancer; Apoptosis
Model: Knock-Out
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Contributor
Inventor: Catrin Pritchard
Institute: University of Leicester
Tool Details
*FOR RESEARCH USE ONLY
- Tool name: Raf KO Mouse
- Tool sub type: Mouse
- Disease: Cancer; Apoptosis
- Model: Knock-Out
- Conditional: No
- Description: The Raf-/- embryo phenotype shows defects in vascularisation and placental development as well as increased apoptosis of many tissues.
- Genetic background: Raf-1 targeting vectors, where exons 10-13 were replaced with a resistance marker, were transfected into 129Ola ES cells. Successfully targeted ES cells containing homologous recombination events were selected and injected into blastocysts, Chimeric offspring were mated to MF-1 outbred background and the the C57BL6 inbred strain.
- Phenotype: Homozygous Raf-1-/- mice die in embryogenesis.
- Zygosity: Heterozygous
- Production details: Raf-1 targeting vectors, where exons 10-13 were replaced with a resistance marker, were transfected into 129Ola ES cells. Successfully targeted ES cells containing homologous recombination events were selected and injected into blastocysts, Chimeric offspring were mated to MF-1 outbred background and the the C57BL6 inbred strain.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Raf1+/- heterozygous knockout
Related Tools
- Related tools: MEF RAF1 KO Cell Line
References
- H
ser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227. - MEK kinase activity is not necessary for Raf-1 function.
