Cat. #151463
Omega-E Packaging Cell Line
Cat. #: 151463
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Mouse
Tissue: Embryo
Model: Packaging
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Hartmut Land
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY
- Name: Omega-E Packaging Cell Line
- Tool sub type: Continuous
- Parental cell: NIH/3T3
- Organism: Mouse
- Tissue: Embryo
- Growth properties: Recombinant retroviral production
- Model: Packaging
- Conditional: Yes
- Description: The Omega-E Packaging Cell Line ias an helper-free packaging cell line & shuttle vector system enabling high-titer production of recombinant retroviral vectors for mammalian gene transfer, with significantly reduced probability of replication-competent retrovirus generation. The pBabe series of vectors represent a highly efficient retroviral based gene transfer system for stable expression within mammalian cells. They are derived from the Moloney murine leukemia virus (MMLV) and can be used ...
- Production details: The Omega E and pBabe recombinant retroviral system utilises shuttle vectors with multiple drug selection markers (the pBabe vector series) for increased flexibility of gene transfer vector design, and a third generation, helper-free packaging cell line (Omega E) derived from mouse NIH 3T3 cell lines, enabling high-titer production of Moloney murine leukemia virus for use in mammalian gene transfer
- Cellosaurus id: CVCL_2151
Target Details
- Target: pBabe vector
Handling
- Format: Frozen
- Growth medium: For recommended growth and recombinant retrovirus production conditions, see Morgenstern & Land. 1990. Nucleic Acids Research. 18:3587-96. PMID: 2194165
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Littlewood et al. 1995. Nucleic Acids Res. 23(10):1686-90. PMID: 7784172.
- A modified oestrogen receptor ligand-binding domain as an improved switch for the regulation of heterologous proteins.
- Morgenstern et al. 1990. Nucleic Acids Res. 18(12):3587-96. PMID: 2194165.
- Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line.