#151463

Omega-E Packaging Cell Line

Cat. #151463

Omega-E Packaging Cell Line

Cat. #: 151463

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 10-12 weeks

Organism: Mouse

Tissue: Embryo

Model: Packaging

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Hartmut Land

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Omega-E Packaging Cell Line
  • Tool sub type: Continuous
  • Parental cell: NIH/3T3
  • Organism: Mouse
  • Tissue: Embryo
  • Growth properties: Recombinant retroviral production
  • Model: Packaging
  • Conditional: Yes
  • Description: The Omega-E Packaging Cell Line ias an helper-free packaging cell line & shuttle vector system enabling high-titer production of recombinant retroviral vectors for mammalian gene transfer, with significantly reduced probability of replication-competent retrovirus generation. The pBabe series of vectors represent a highly efficient retroviral based gene transfer system for stable expression within mammalian cells. They are derived from the Moloney murine leukemia virus (MMLV) and can be used ...
  • Production details: The Omega E and pBabe recombinant retroviral system utilises shuttle vectors with multiple drug selection markers (the pBabe vector series) for increased flexibility of gene transfer vector design, and a third generation, helper-free packaging cell line (Omega E) derived from mouse NIH 3T3 cell lines, enabling high-titer production of Moloney murine leukemia virus for use in mammalian gene transfer
  • Cellosaurus id: CVCL_2151

Target Details

  • Target: pBabe vector

Handling

  • Format: Frozen
  • Growth medium: For recommended growth and recombinant retrovirus production conditions, see Morgenstern & Land. 1990. Nucleic Acids Research. 18:3587-96. PMID: 2194165
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Littlewood et al. 1995. Nucleic Acids Res. 23(10):1686-90. PMID: 7784172.
  • A modified oestrogen receptor ligand-binding domain as an improved switch for the regulation of heterologous proteins.
  • Morgenstern et al. 1990. Nucleic Acids Res. 18(12):3587-96. PMID: 2194165.
  • Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line.