Cat. #151462
Anti-CHD4 [CHD4 3F2/4]
Cat. #: 151462
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: Chromodomain-helicase-DNA-binding protein 4 (CHD4/Mi2b)
Class: Monoclonal
Application: ChIP ; ELISA ; FACS ; IF ; IHC ; IP ; WB
Reactivity: Mouse ; Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-CHD4 [CHD4 3F2/4]
- Clone: CHD4 3F2/4
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: CD1
- Reactivity: Mouse ; Human
- Host: Mouse
- Application: ChIP ; ELISA ; FACS ; IF ; IHC ; IP ; WB
- Description: CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.
- Immunogen: Synthetic peptide (N- and C- terminal peptides were used for immunisation. 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ. Which peptide the antibody recognises has not been tested)
- Isotype: IgG2a
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: Chromodomain-helicase-DNA-binding protein 4 (CHD4/Mi2b)
- Target background: CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.
Applications
- Application: ChIP ; ELISA ; FACS ; IF ; IHC ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -80° C
- Shipping conditions: Shipping at 4° C
References
- Sparmann et al. 2013. EMBO J. 32(11):1598-612. PMID: 23624931.
- The chromodomain helicase Chd4 is required for Polycomb-mediated inhibition of astroglial differentiation.
- Curtis et al. 2012. Mol Cell Biol. 32(7):1312-20. PMID: 22290435.
- The chromatin-remodeling enzymes BRG1 and CHD4 antagonistically regulate vascular Wnt signaling.
- Ram
rez et al. 2009. Epigenetics. 4(8):532-6. PMID: 19923891. - The Mi-2/NuRD complex: a critical epigenetic regulator of hematopoietic development, differentiation and cancer.
