Cat. #151442
Inva5b1 Mouse
Cat. #: 151442
Sub-type: Mouse
Availability: 6-8 weeks
Disease: Psoriasis
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Contributor
Inventor: Fiona Watt
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY
- Tool name: Inva5b1 Mouse
- Tool sub type: Mouse
- Disease: Psoriasis
- Conditional: Yes
- Conditional description: Conditional transgenic
- Description: Disease model for psoriasis; in vivo study of human a5 and b1 integrin transgene expression in skin A transgenic mouse expressing integrin a5B1 in the suprabasal layers of the epidermis. The mouse exhibits many of the characteristics associated with psoriasis including hyperproliferation of keratinocytes and infiltration of lymphocytes. Granted patents are associated with this mouse (US6187993 and AU0698317). Alpha5beta1 integrin transgenes under involucrin promoter, giving conditional suprabasal epidermal expression. Psoriasis phenotype
- Genetic background: Involucrin-integrin transgene expression constructs were injected into fertilised oocytes from F1 hybrid CBA/C57BL/6 mice. Transgene-positive mice were back-crossed to generate individual founder lines. The a5 transgene founder line was mated to the b1 integrin founder line to generate mice expressing both a5 and b1 integrin transgenes.
- Phenotype: The Inv-a5b1 mouse presents a phenotype very similar to psoriasis, and is an ideal tool for study of the disease in vivo.Abnormal keratinocyte differentiation - widespread thickening of the epidermisKeratinocyte hyperproliferation - flaking epidermis. High numbers of Ki-67 positive cells in all basal and suprabasal layers- Keratin 6 expression in all epidermal layers- Inflammatory skin lesions - dilated capillaries and reddening of skin- Pustules with neutrophil infiltration within or beneath cornified layers - high numbers of CD4 and CD8 lymphocytes within epidermisThere are a number of other psoriasis disease models please see the related page.
- Production details: Involucrin-integrin transgene expression constructs were injected into fertilised oocytes from F1 hybrid CBA/C57BL/6 mice. Transgene-positive mice were back-crossed to generate individual founder lines. The a5 transgene founder line was mated to the b1 integrin founder line to generate mice expressing both a5 and b1 integrin transgenes.
- Additional notes: The Inv-a5b1 mouse presents a phenotype very similar to psoriasis, and is an ideal tool for study of the disease in vivo. Abnormal keratinocyte differentiation - widespread thickening of the epidermis Keratinocyte hyperproliferation - flaking epidermis. High numbers of Ki-67 positive cells in all basal and suprabasal layers - Keratin 6 expression in all epidermal layers - Inflammatory skin lesions - dilated capillaries and reddening of skin - Pustules with neutrophil infiltration within or beneath cornified layers - high numbers of CD4 and CD8 lymphocytes within epidermis There are a number of other psoriasis disease models please see the related page.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: a5 integrin, b1 integrin
Related Tools
- Related tools: Inva2b1 Mouse ; Inva3b1 Mouse ; InvEE Mouse
References
- Haase et al. 2001. J Clin Invest. 108(4):527-36. PMID: 11518726.
- A role for mitogen-activated protein kinase activation by integrins in the pathogenesis of psoriasis.
- Carroll et al. 1995. Cell. 83(6):957-68. PMID: 8521519.
- Carroll et al. 1995. Cell. 83(6):957-68. PMID: 8521519.
- Suprabasal integrin expression in the epidermis of transgenic mice results in developmental defects and a phenotype resembling psoriasis.
