Anti-nNav 1.5 [4G8:1G7]

Tool Details

*FOR RESEARCH USE ONLY

• Name: Anti-nNav 1.5 [4G8:1G7]
• Clone: 4G8:1G7
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 180-200 kDa
• Strain: CD1
• Reactivity: Human
• Host: Mouse
• Application: FACS ; IHC ; IF ; WB
• Description: Voltage-gated sodium channesl (VGSCs) mediate regenerative cell membrane depolarization and conduction of electrical signalling in nerves and muscles. They have also been detected in lymphocytes, glia, and fibroblasts. VGSC proteins are comprised of a core alpha-subunit, together with one or more additional regulatoy beta-subunit. VCSC expression has been detected in a number of tumour cell lines and has been implicated in the metastatic behaviour of breast and prostate cancer cells. Na(v)1.5, the major cardiac voltage-gated Na(+) channel, plays a central role in the generation of the cardiac action potential and in the propagation of electrical impulses in the heart. Its importance for normal heart function has been recently exemplified by reports of numerous mutations found in the gene SCN5A which encodes Na(v)1.5.
• Immunogen: Peptide (14mer) from an extracellular domain of Nav1.5 - VSENIKLGNLSALR
• Isotype: IgM
• Myeloma used: Sp2/0-Ag14
• Recommended controls: Cell lines over expressing Nav1.5, e.g Nav 1.5-HEK293 cells

Target Details

• Target: Neonatal Nav1.5 (Voltage gated Sodium Channel Nav1.5)
• Molecular weight: 180-200 kDa
• Tissue cell line specificity: Cell lines over expressing Nav1.5, e.g Nav 1.5-HEK293 cells
• Target background: Voltage-gated sodium channesl (VGSCs) mediate regenerative cell membrane depolarization and conduction of electrical signalling in nerves and muscles. They have also been detected in lymphocytes, glia, and fibroblasts. VGSC proteins are comprised of a core alpha-subunit, together with one or more additional regulatoy beta-subunit. VCSC expression has been detected in a number of tumour cell lines and has been implicated in the metastatic behaviour of breast and prostate cancer cells. Na(v)1.5, the major cardiac voltage-gated Na(+) channel, plays a central role in the generation of the cardiac action potential and in the propagation of electrical impulses in the heart. Its importance for normal heart function has been recently exemplified by reports of numerous mutations found in the gene SCN5A which encodes Na(v)1.5.

Applications

• Application: FACS ; IHC ; IF ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Vikram et al. 2017. Nat Med. 23(3):361-367. PMID: 28191886.
• Utrilla et al. 2017. Front Physiol. 8:903. PMID: 29184507.
• Guo et al. 2011. Toxicol Sci. 123(1):281-9. PMID: 21693436.
• Guo et al. 2011. Toxicol Sci. 123(1):281-9. PMID: 21693436.
• Estimating the risk of drug-induced proarrhythmia using human induced pluripotent stem cell-derived cardiomyocytes.