Anti-TCP1 [23c (CTA-123)]

Tool Details

*FOR RESEARCH USE ONLY

• Name: Anti-TCP1 [23c (CTA-123)]
• Clone: 23c (CTA-123)
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Chicken ; Dog ; Hamster ; Mouse ; Rat
• Host: Rat
• Application: IHC ; IF ; IP ; WB
• Description: TCP1, (a constituent of chaperone-containing TCP-1, CCT) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells and upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin.
• Immunogen: C-terminal half of the full length murine TCP protein
• Isotype: IgG2c
• Myeloma used: Y3.AG.1.2.3

Target Details

• Target: TCP1 (T-complex polypeptide 1)
• Target background: TCP1, (a constituent of chaperone-containing TCP-1, CCT) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells and upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin.

Applications

• Application: IHC ; IF ; IP ; WB

Handling

• Format: Liquid
• Concentration: 0.9-1.1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Lobanova K et al, 2013. PNAS 110(24):9986-91 PMID 23716657
• Sternlicht et al. 1993. Proc Natl Acad Sci U S A. 90(20):9422-6. PMID: 8105476.
• The t-complex polypeptide 1 complex is a chaperonin for tubulin and actin in vivo.
• Harrison-Lavoie et al. 1993. EMBO J. 12(7):2847-53. PMID: 8335000.
• A 102 kDa subunit of a Golgi-associated particle has homology to beta subunits of trimeric G proteins.
• Willison et al. 1989. Cell. 57(4):621-32. PMID: 2655925.
• The t complex polypeptide 1 (TCP-1) is associated with the cytoplasmic aspect of Golgi membranes.