Cat. #151263
Anti-HIF2A [EP190b]
Cat. #: 151263
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: Hypoxia-inducible factor 2, alpha subunit (HIF2A)
Class: Monoclonal
Application: ELISA ; IHC ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Helen Turley
Institute: University of Oxford
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-HIF2A [EP190b]
- Clone: EP190b
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; IHC ; WB
- Description: Induction of the Hif regulated genes, as a consequence of the microenvironment or genetic changes, is known to have an important role in the growth of experimental tumours. HIF2A is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF1A has been observed in varying subsets of tumour cells from various solid tumours. HIF2A is also a potent activator of the tie2 gene, which is known to be selectively expressed in endothelial cells.
- Immunogen: GST-human EPAS-1 (HIF2A) amino acids 535-631 fusion protein
- Isotype: IgG1
- Myeloma used: P3/NS1/1-Ag4.1
Target Details
- Target: Hypoxia-inducible factor 2, alpha subunit (HIF2A)
- Target background: Induction of the Hif regulated genes, as a consequence of the microenvironment or genetic changes, is known to have an important role in the growth of experimental tumours. HIF2A is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF1A has been observed in varying subsets of tumour cells from various solid tumours. HIF2A is also a potent activator of the tie2 gene, which is known to be selectively expressed in endothelial cells.
Applications
- Application: ELISA ; IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
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- Giatromanolaki et al. 2003. J Pathol. 200(2):222-8. PMID: 12754744.
- DEC1 (STRA13) protein expression relates to hypoxia- inducible factor 1-alpha and carbonic anhydrase-9 overexpression in non-small cell lung cancer.
- Hui et al. 2002. Clin Cancer Res. 8(8):2595-604. PMID: 12171890.
- Coexpression of hypoxia-inducible factors 1alpha and 2alpha, carbonic anhydrase IX, and vascular endothelial growth factor in nasopharyngeal carcinoma and relationship to survival.
- Beasley et al. 2002. Cancer Res. 62(9):2493-7. PMID: 11980639.
- Hypoxia-inducible factors HIF-1alpha and HIF-2alpha in head and neck cancer: relationship to tumor biology and treatment outcome in surgically resected patients.
- Talks et al. 2000. Am J Pathol. 157(2):411-21. PMID: 10934146.
- The expression and distribution of the hypoxia-inducible factors HIF-1alpha and HIF-2alpha in normal human tissues, cancers, and tumor-associated macrophages.
- Wiesener et al. 1998. Blood. 92(7):2260-8. PMID: 9746763.
- Induction of endothelial PAS domain protein-1 by hypoxia: characterization and comparison with hypoxia-inducible factor-1alpha.
