Cat. #151243
Anti-Phospho RNA polII (Ser5) [CTD 8A7]
Cat. #: 151243
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: RNA Polymerase II (Phosphorylated)
Class: Monoclonal
Application: IF ; ELISA ; FACS ; IF ; WB
Reactivity: Human ; Saccharomyces cerevisiae
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Jesper Svejstrup
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-Phospho RNA polII (Ser5) [CTD 8A7]
- Clone: CTD 8A7
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Human ; Saccharomyces cerevisiae
- Host: Mouse
- Application: IF ; ELISA ; FACS ; IF ; WB
- Description: RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.
- Immunogen: Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically sythesized phospho-Ser5.
- Isotype: IgM
- Myeloma used: Sp2/0-Ag14
- Recommended controls: S. cerevisiae and HeLa cells
Target Details
- Target: RNA Polymerase II (Phosphorylated)
- Tissue cell line specificity: S. cerevisiae and HeLa cells
- Target background: RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.
Applications
- Application: IF ; ELISA ; FACS ; IF ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
Related Tools
- Related tools: Anti-RNA polII [CTD 4H8]
References
- Szafran et al. 2017. PLoS One. 12(7):e0180141. PMID: 28704378.
- Trevi
o et al. 2016. Mol Endocrinol. 30(2):158-72. PMID: 26652902. - Benferhat et al. 2012. J Virol. 86(20):11333-44. PMID: 22896612.
- Large-scale chromatin immunoprecipitation with promoter sequence microarray analysis of the interaction of the NSs protein of Rift Valley fever virus with regulatory DNA regions of the host genome.
- Alm-Kristiansen et al. 2011. Mol Cancer. 10:21. PMID: 21338522.
- PIAS1 interacts with FLASH and enhances its co-activation of c-Myb.
- Aune et al. 2008. Clin Cancer Res. 14(20):6449-55. PMID: 18927284.
- Von Hippel-Lindau-coupled and transcription-coupled nucleotide excision repair-dependent degradation of RNA polymerase II in response to trabectedin.
- Otero et al. 1999. Mol Cell. 3(1):109-18. PMID: 10024884.
- Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation.
