Cat. #151240
Anti-S100A8 [7C12/4]
Cat. #: 151240
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: S100A8 (MRP8)
Class: Monoclonal
Application: ELISA ; FACS ; IHC ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Nancy Hogg
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-S100A8 [7C12/4]
- Clone: 7C12/4
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; FACS ; IHC ; WB
- Description: S100A8 and S100A9 are cytosolic calcium-binding proteins of 8kDa and 14kDa respectively that form a heterodimer. S100A8 and S100A9 are expressed in peripheral blood monocytes, neutrophils and keratinocytes (mature). Expression is lost on tissue maturation of monocytes to macrophages. S100A9 may be associated with monocyte and neutrophil activation and the accumulation of these cells in inflammatory sites.
- Immunogen: Recombinant human S100A8
- Isotype: IgM
- Myeloma used: Sp2/0-Ag14
- Recommended controls: Neutrophil lysate (from spleen)
Target Details
- Target: S100A8 (MRP8)
- Tissue cell line specificity: Neutrophil lysate (from spleen)
- Target background: S100A8 and S100A9 are cytosolic calcium-binding proteins of 8kDa and 14kDa respectively that form a heterodimer. S100A8 and S100A9 are expressed in peripheral blood monocytes, neutrophils and keratinocytes (mature). Expression is lost on tissue maturation of monocytes to macrophages. S100A9 may be associated with monocyte and neutrophil activation and the accumulation of these cells in inflammatory sites.
Applications
- Application: ELISA ; FACS ; IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Robinson et al. 2002. J Biol Chem. 277(5):3658-65. PMID: 11723110.
- The S100 family heterodimer, MRP-8/14, binds with high affinity to heparin and heparan sulfate glycosaminoglycans on endothelial cells.
