#151218

Anti-Integrin a4 [7.2]

Cat. #151218

Anti-Integrin a4 [7.2]

Cat. #: 151218

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Integrin alpha 4 (VLA-4; CD49d)

Class: Monoclonal

Application: FACS ; IHC ; IP ; WB

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: John Marshall

Institute: Queen Mary University of London

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-Integrin a4 [7.2]
  • Clone: 7.2
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS ; IHC ; IP ; WB
  • Description: Monoclonal antibody directed at integrin a4/b1, which forms part of VLA-4.
  • Immunogen: Human melanoma line DX3
  • Immunogen uniprot id: P13612
  • Isotype: IgG1
  • Recommended controls: U937 human histiocytic lymphoma cell line

Target Details

  • Target: Integrin alpha 4 (VLA-4; CD49d)
  • Tissue cell line specificity: U937 human histiocytic lymphoma cell line
  • Target background: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin alpha 4 combines with beta-1 integrin to form the Integrin alpha4/ beta1 lymphocyte homing receptor: Very Late Antigen 4 (VLA-4). VLA-4 plays a major role in lymphocyte extravasation, via binding to VCAM on endothelial cells. VLA-4 also binds fibronectin and mediates homotypic and heterotypic cell-cell adhesion between lymphocytes. Integrin alpha 4/ beta 1 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1).

Applications

  • Application: FACS ; IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4° C

References

  • McDowall et al. 2003. J Clin Invest. 111(1):51-60. PMID: 12511588.
  • A novel form of integrin dysfunction involving beta1, beta2, and beta3 integrins.
  • Leitinger et al. 2002. J Cell Sci. 115(Pt 5):963-72. PMID: 11870215.
  • The involvement of lipid rafts in the regulation of integrin function.