Cat. #151210
Anti-Cig2 [CIG2 3A11/5]
Cat. #: 151210
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Cig2
Class: Monoclonal
Application: ELISA ; IHC ; IF ; IP ; WB
Reactivity: Schizosaccharomyces pombe
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-Cig2 [CIG2 3A11/5]
- Alternate name: Cyc17
- Clone: CIG2 3A11/5
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Schizosaccharomyces pombe
- Host: Mouse
- Application: ELISA ; IHC ; IF ; IP ; WB
- Description: Cig2 is a fission yeast (S. pombe) B-type S-phase cyclin and is expressed in a sharp spike peaking at G1/S period. Cig2 binds to Cdc2 (Cdk1), and the resulting Cdc2-Cig2 complex controls the G1/S transition of the cell cycle.
- Immunogen: S. pombe Cig2 purified protein (bacterial expression)
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: Cig2
- Target background: Cig2 is a fission yeast (S. pombe) B-type S-phase cyclin and is expressed in a sharp spike peaking at G1/S period. Cig2 binds to Cdc2 (Cdk1), and the resulting Cdc2-Cig2 complex controls the G1/S transition of the cell cycle.
Applications
- Application: ELISA ; IHC ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Chu et al. 2009. Mol Cell Biol. 29(18):4959-70. PMID: 19596787.
- A Cds1-mediated checkpoint protects the MBF activator Rep2 from ubiquitination by anaphase-promoting complex/cyclosome-Ste9 at S-phase arrest in fission yeast.
- Chu et al. 2007. Mol Biol Cell. 18(5):1756-67. PMID: 17332498.
- Modulation of cell cycle-specific gene expressions at the onset of S phase arrest contributes to the robust DNA replication checkpoint response in fission yeast.
- Yamano et al. 2000. Mol Cell. 6(6):1377-87. PMID: 11163211.
- The spike of S phase cyclin Cig2 expression at the G1-S border in fission yeast requires both APC and SCF ubiquitin ligases.
