#151180

Anti-CyclinB1 [V92.1]

Cat. #151180

Anti-CyclinB1 [V92.1]

Cat. #: 151180

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Cyclin B1

Class: Monoclonal

Application: FACS ; IHC ; IF ; IP ; WB

Reactivity: Hamster ; Human ; Mouse

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Julian Gannon

Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-CyclinB1 [V92.1]
  • Alternate name: CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1Human Entrez Gene ID 891 Human SwissProt P14635 Human Unigene 2396 Human Gene Symbol CCNB1Human Chromosome Location 5q13.2
  • Clone: V92.1
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 55-62 kDa
  • Reactivity: Hamster ; Human ; Mouse
  • Host: Mouse
  • Application: FACS ; IHC ; IF ; IP ; WB
  • Description: Clone V92.1, recognizes a protein of 55-62kDa, identified as cyclin B1.
  • Immunogen: Hamster Cyclin B1
  • Isotype: IgG1 kappa
  • Myeloma used: Sp2/0-Ag14
  • Recommended controls: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.

Target Details

  • Target: Cyclin B1
  • Molecular weight: 55-62 kDa
  • Tissue cell line specificity: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.
  • Target background: Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin B1 is a marker of cell proliferation and a key component of the cell cycle progression machinery. Cyclin B1 may prove to be a prognostic marker in neoplasia. V92.1 may be used to immunoprecipitate active CDK1/cyclin complexes.

Applications

  • Application: FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Garner et al. 2002. Oncogene. 21(53):8089-104. PMID: 12444545.
  • Delta MEKK3:ER* activation induces a p38 alpha/beta 2-dependent cell cycle arrest at the G2 checkpoint.
  • Minemoto et al. 2001. Exp Cell Res. 262(1):37-48. PMID: 11120603.
  • Characterization of adriamycin-induced G2 arrest and its abrogation by caffeine in FL-amnion cells with or without p53.