Anti-Keratin7,13,17 & 18 [LdS 23]

Tool Details

*FOR RESEARCH USE ONLY

• Name: Anti-Keratin7,13,17 & 18 [LdS 23]
• Clone: LdS 23
• Tool type ecom: Antibodies
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Strain: Balb/c
• Reactivity: Human
• Host: Mouse
• Application: IHC ; IF ; IP ; WB
• Description: LdS 23 is useful for studies of epithelial cells and tumour origin.
• Immunogen: Cytoskeletons of the human urothelial RT4 cell line
• Isotype: IgG1 kappa
• Myeloma used: Sp2/0-Ag14

Target Details

• Target: Keratin 7,13,17 & 18
• Target background: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue- and differentiation-specific expression profiles. Keratins 7 and 8 are two closely related type II keratins characteristic of simple epithelia. Keratin 7 is less widespread than keratin 8 and is expressed in sebaceous and sweat glands and some cells of the inner hair root sheath. Keratin 7 is often co-expressed with keratin 19. Keratin 17 is expressed in suprabasal keratinocytes of wounded epidermis. Keratins 8 and 18 are two of the first keratins expressed in the embryo, and persist into adult tissues as the keratin pair representing minimal epithelial keratin expression. Keratins 8 and 18 are major components of all simple epithelia (but not of stratified squamous epithelia) and adenocarcinomas.

Applications

• Application: IHC ; IF ; IP ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Trejdosiewicz et al. 1986. Exp Cell Res. 164(2):388-98. PMID: 2423348.
• Phenotypic analysis of cultured melanoma cells. Expression of cytokeratin-type intermediate filaments by the M5 human melanoma cell line.