Cat. #151088
Anti-CyclinA [E23.1]
Cat. #: 151088
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: Cyclin A
Class: Monoclonal
Application: FACS ; IHC ; IF ; IP ; WB
Reactivity: Mammalian
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Julian Gannon
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-CyclinA [E23.1]
- Alternate name: CCNA1; Cyclin A1; Testicular Tissue Protein Li 34; CT146
- Clone: E23.1
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Mammalian
- Host: Mouse
- Application: FACS ; IHC ; IF ; IP ; WB
- Description: E23.1 is extremely sensitive for immunoblotting Cyclin A.
- Immunogen: Bovine Cyclin A fusion protein.
- Immunogen uniprot id: P17927
- Isotype: IgG2a
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: Cyclin A
- Target background: Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin A is involved in the regulation of the cell cycle and is essential for progression through S phase. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is a marker for actively proliferating cells and for cells in S phase.
Applications
- Application: FACS ; IHC ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Hagen et al. 2013. Cell Div. 8(1):10. PMID: 23886499.
- Silencing CDK4 radiosensitizes breast cancer cells by promoting apoptosis.
- Li et al. 2012. Proc Natl Acad Sci U S A. 109(31):12550-5. PMID: 22802651.
- Gap 1 phase length and mouse embryonic stem cell self-renewal.
- Hogg et al. 2011. Free Radic Biol Med. 50(9):1065-74. PMID: 21256959.
- Effect of nitric oxide on neointimal hyperplasia based on sex and hormone status.
- Geley et al. 2001. J Cell Biol. 153(1):137-48. PMID: 11285280.
- Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint.
- Adamczewski et al. 1993. J Virol. 67(11):6551-7. PMID: 8411358.
- Simian virus 40 large T antigen associates with cyclin A and p33cdk2.
