#151073

Anti-APC [Ali 12-28]

Cat. #151073

Anti-APC [Ali 12-28]

Cat. #: 151073

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: Adenomatous Polyposis Coli (APC)

Class: Monoclonal

Application: WB ; ELISA ; IHC ; IF ; IP ; WB

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Walter Bodmer

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-APC [Ali 12-28]
  • Clone: Ali 12-28
  • Tool type ecom: Antibodies
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 310 kDa
  • Strain: Balb/c
  • Reactivity: Human
  • Host: Mouse
  • Application: WB ; ELISA ; IHC ; IF ; IP ; WB
  • Description: Ali 12-28 may be used for the detection of APC expression and APC mutations.
  • Immunogen: N-terminal APC-MBP fusion protein
  • Isotype: IgG1
  • Myeloma used: Sp2/0-Ag14
  • Recommended controls: Cancer cell lines: SW480, LOVO, DLD1, SW837

Target Details

  • Target: Adenomatous Polyposis Coli (APC)
  • Molecular weight: 310 kDa
  • Tissue cell line specificity: Cancer cell lines: SW480, LOVO, DLD1, SW837
  • Target background: The adenomatous polyposis coli (APC) tumour suppressor gene is mutated (often by deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and in over 80% of colorectal cancers.

Applications

  • Application: WB ; ELISA ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Hadjihannas et al. 2012. EMBO Rep. 13(4):347-54. PMID: 22322943.
  • Cell cycle control of Wnt/?-catenin signalling by conductin/axin2 through CDC20.
  • Valvezan et al. 2012. J Biol Chem. 287(6):3823-32. PMID: 22184111.
  • Tanneberger et al. 2011. J Biol Chem. 286(22):19204-14. PMID: 21498506.
  • Structural and Fn characterization of the Wnt inhibitor APC membrane recruitment 1 (Amer1).
  • Arnold et al. 2009. EMBO J. 28(5):500-12. PMID: 19131971.
  • The Axin1 scaffold protein promotes formation of a degradation complex for c-Myc.
  • Efstathiou et al. 1998. Proc Natl Acad Sci U S A. 95(6):3122-7. PMID: 9501226.
  • Intestinal trefoil factor controls the expression of the adenomatous polyposis coli-catenin and the E-cadherin-catenin complexes in human colon carcinoma cells.