#151066

Anti-Blood group A sugar determinant [9A]

Cat. #151066

Anti-Blood group A sugar determinant [9A]

Cat. #: 151066

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Blood group A sugar determinant

Class: Monoclonal

Application: FACS ; ELISA ; IHC ; IP

Reactivity: Human

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Peter Parker

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-Blood group A sugar determinant [9A]
  • Clone: 9A
  • Tool type ecom: Antibodies
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 52 kDa
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS ; ELISA ; IHC ; IP
  • Description: 9A is suitable for binding to the EGF receptor in A431 cells only.
  • Immunogen: A431 cells (human vulval squamous carcinoma cell line)
  • Isotype: IgG3
  • Myeloma used: P3X63Ag8

Target Details

  • Target: Blood group A sugar determinant
  • Molecular weight: 52 kDa
  • Target background: 9A is suitable for binding to the EGF receptor in A431 cells only. These cells have a glycosylation defect which reveals otherwise cryptic sugar structures.

Applications

  • Application: FACS ; ELISA ; IHC ; IP

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

References

  • Increased a-series gangliosides positively regulate leptin/Ob receptor-mediated signals in hypothalamus of GD3 synthase-deficient mice.
  • Fischer et al. 2015. Front Immunol. 6:211. PMID: 25999949.
  • Gullick et al. 1990. J Pathol. 162(3):275-6. PMID: 2266467.
  • 'EGF receptor' antibody 29.1.1.
  • Parker et al. 1984. J Biol Chem. 259(15):9906-12. PMID: 6086658.
  • Monoclonal antibodies against the human epidermal growth factor receptor from A431 cells. Isolation, characterization, and use in the purification of active epidermal growth factor receptor.