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UK Health Security Agency

HeLa EGFP-Histone H2B

HeLa EGFP-Histone H2B

Catalogue No.

17100529

Cell Line Name

HeLa EGFP-Histone H2B

Cell Line Description

The human histone H2B gene was fused to the gene encoding the enhanced green fluorescent protein (EGFP) and transfected into human HeLa cells to generate a stable line constitutively expressing H2B-GFP. The H2B-GFP fusion protein was incorporated into nucleosomes without affecting cell cycle progression.

General Info

Species

Human

Unique to ECACC

Yes

Also Known As

HeLa S3 EGFP-H2B

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Tissue of Origin

Cervix

Morphology

Adherent epithelial cells

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 9,10
D3S1358: 15,18
D5S818: 11,12
D7S820: 8,12
D8S1179: 12,13
D13S317: 12,13.3
D16S539: 9,10
D18S51: 15,16
D21S11: 27,28
FGA: 18,21
Penta D: 8,15
Penta E: 7,17
TH01: 7
TPOX: 8,12
vWA: 16,18

Disease

Adenocarcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Trypsinise cultures at 80% confluence with 0.05% Trypsin/EDTA. Seed flasks at 1-3 x10⁴ cells/cm². Cultures must be incubated in a humidified 10% CO₂/90% air incubator at 37°C. Note: The depositor had grown these cells at 5% CO₂. Here at ECACC we grow cells at 10% CO₂ when cells are grown in DMEM media.

Culture Medium

Dulbecco's Modified Eagle's Medium, high glucose (DMEM) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Recommend used of selection antibiotics (4µg/ml Blastocidin) for maintenance of expression. The transgene is stably integrated into the genome of the cells so for short term culture it is not strictly necessary to include the antibiotics.

Growth Mode

Adherent

Additional Info

Depositor

Ximbio (formerly known as Cancer Research Technologies Limited)

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Zeng., K. et al Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2. J Cell Biol. 2019 (Mar).191(7): 1315-1332. phttp://doi.org/10.1083/jcb.201008106. PMID: 21187329 PMCID: PMC3010072

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: HeLa EGFP-Histone H2B (ECACC 17100529).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.