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S_N40R2

S_N40R2

Catalogue No.

17011210

Cell Line Name

S_N40R2

Cell Line Description

S_N40R2 is a subpopulation of the SJSA-1 osteosarcoma line which is resistant to 40 uM Nutlin-3.

General Info

Species

Human

Unique to ECACC

Yes

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Receptors

There is an amplification of the gene which encodes the p53 protein, MDM2. These cells also exhibit a 15-fold amplification of the gli proto-oncogene

Tissue of Origin

Bone

Morphology

Fibroblast

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 7.3,10.1
D3S1358: 16,17
D5S818: 12
D7S820: 9,11
D8S1179: 13,15
D13S317: 12
D16S539: 9,10
D18S51: 15,17
D21S11: 28,33.2
FGA: 22,23
Penta D: 2.2,10
Penta E: 11,14
THO: 7,9
TPOX: 8,9
vWA: 19

Disease

Osteosarcoma

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10⁴ cells/cm² using 0.05% trypsin/EDTA solution. Cells are cultured at 37°C, in 95% air and 5% CO₂, and passaged every 3 to 4 daysFreeze medium: Foetal Bovine Serum (FBS) 90% and DMSO 10%

Culture Medium

RPMI 1640+10% FBS

Growth Mode

Adherent

Additional Info

Depositor

Ximbio (formerly known as Cancer Research Technologies Limited)

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Drummond CJ, Esfandiari A, Liu J, Lu X, Hutton C, Jackson J, Bennaceur K, Xu Q, Makimanejavali AR, Del Bello F, Piergentili A, Newell DR, Hardcastle IR, Griffin RJ, Lunec J.TP53 mutant MDM2-amplified cell lines selected for resistance to MDM2-p53 binding antagonists retain sensitivity to ionizing radiation. Oncotarget. 2016 Jul 19;7(29):46203-46218. doi: 10.18632/oncotarget PMID: 27323823

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: S_N40R2 (ECACC 17011210).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.