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UK Health Security Agency

T47D-182R2

T47D-182R2

Catalogue No.

16022508

Cell Line Name

T47D-182R2

Cell Line Description

The T47D-182R2 cell line is a breast cancer cell line resistant to fulvestrant. It is derived from the human breast cancer cell line derived from T47D/S5 by long term treatment with 100 nM fulvestrant.

General Info

Species

Human

Unique to ECACC

Yes

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Receptors

After growth in medium containing with 5% foetal bovine serum and 100 nM fulvestrant the cells are oestrogen and progesterone receptor negative.

Tissue of Origin

Breast

Morphology

Epithelial

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,13
D3S1358: 15,17
D5S818: 12
D7S820: 11
D8S1179: 13
D13S317: 12
D16S539: 10
D18S51: 17
D21S11: 28,31
FGA: 23
Penta D: 10,12
Penta E: 7,14
TH01: 6
TPOX: 11
vWA: 14

Applications

The T47D-182R2 cell line is a breast cancer cell line resistant to fulvestrant. Treatment with the steroidal anti-oestrogen fulvestrant has proven effective upon progression on tamoxifen therapy and is now approved for second-line treatment after tamoxifen or aromatase inhibitors. As for tamoxifen treatment of advanced breast cancer, resistance will inevitably occur also for fulvestrant. Clarification of the molecular changes associated with the resistant growth is needed to find targeted treatments to resistant tumour cells and treatments that can inhibit or delay the emergence of resistance.

Disease

Pleural Effusion of a Ductal Carcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures at 70-80% confluency. Subculture cells using 0.05% trypsin/EDTA solution and soya bean trypsin inhibitor or TrypLE Express™ (1X), without phenol red, and wash with phosphate buffered saline (PBS). Pellet cells by centrifugation and re-suspend in growth medium. Initiate the culture at a dilution of approximately 1:3 to 1:6 i.e. seeding at 2-4 x 10⁵ cells per cm². Incubate cultures in 5% CO₂ in air at 37°C. Recommend media changes every 2-3 days. Cells can be cryopreserved in either a mixture of cell line conditioned medium (45%) and fresh serum free-medium (45%) with 10% dimethyl sulphoxide (DMSO) or 90% foetal bovine serum (FBS) and 10% dimethyl sulphoxide (DMSO). It is recommended that upon resuscitation the cells are washed in phosphate buffered saline (PBS) or serum-free medium, pelleted by centrifugation and re-suspended in growth medium prior to initiation of culture.

Culture Medium

Phenol red free RPMI 1640 + 5% FBS + Glutamax + 8µg Insulin/ml + 100 nM fulvestrant.

Growth Mode

Adherent

Additional Info

Depositor

Ximbio (formerly known as Cancer Research Technologies Limited)

Country of Origin

Denmark

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: T47D-182R2 (ECACC 16022508).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.