United Kingdom (UK)
Recombinant monoclonal antibody to CaMPARI-Red. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 4F6.
Alternative Name(s) of Target: CaMPARI 1&2; CaMPARI1; CaMPARI2; CaMPARI-Red; Calcium Modulated Photoactivatable Ratiometric Integrator; 4F6-1
Immunogen: This antibody was originally raised by immunizing mice with a purified and photoconverted EosFP protein, subjected it to proteolysis, and further purified the proteolytic fragment containing the red chromophore which is identical to the equivalent region of CaMPARI1 and CaMPARI2.
Specificity: This antibody is specific for the photoconverted form of CaMPARI-Red (both CaMPARI1 and CaMPARI2). CaMPARI is s a photoconvertible protein construct that allows imaging of the integrated calcium activity of populations of cells over time.
Application Notes: 4F6 is recommended for the detection and analysis of CaMPARI via immunohistochemistry (IHC) and Western blot (WB) (Moeyaert et al., 2018; PMID: 30361563). For instance, it was successfully used in the immunohistochemical identification of CaMPARI-Red cells in brain tissue, distinguishing them from the cells in which CaMPARI stayed in ints green, unconverted form (Ebner et al., 2019; PMID: 31178713). This product (Ab01649-23.0) was used for indirect immunofluorescence (IF) staining of adult female Drosophila fly brains to visualize and detect the localization of the CaMPARI-Red protein (Gendron et al., 2023; PMID: 37310911).
Antibody first published in:
Moeyaert et al. Improved methods for marking active neuron populations Nat Commun. 2018 Oct 25;9(1):4440. doi: 10.1038/s41467-018-06935-2 PMID:30361563
Note on publication:
The original publication presents the development of CaMPARI2, an improved fluorescent protein sensor with enhanced properties for marking active neuronal populations, along with the introduction of an immunohistochemical detection method using an anti-CaMPARI-red antibody for selective labeling of photoconverted CaMPARI in activated neurons in rodent brain tissue.