Anti-IgT [N5P2D2*B4]

(Cat#: 153304)
Description
Cat# 153304
Applications ELISA, FACS, IHC, WB
Antigen/Gene or Protein Targets IgT, Immunoglobulin Heavy Constant Tau
Synonyms IgT
Reactivity Salmon and Rainbow Trout
Relevance The IgT isotype, which is unique for teleosts, appears to be specialized to mucosal immune responses.
Host Mouse
Immunogen Ovalbumin-conjugated synthetic peptides- N5 peptide
Positive Control

ELISA: immunized fish sera

Western Blot: recombinant IgT, immunized fish sera

FACS: head kidney

IHC: formalin-fixed, paraffin-embedded PKD infected fish kidney

Subclass IgG1
Myeloma Used P3X63Ag8.653
Strain Balb/c
Notes Recommended Usage Conditions: ELISA - neat; Western blot - neat; FACS - 1/2 dilution; IHC - 1/5 dilution, antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0
Research Area Aquaculture

Image: Immunohistochemistry image using Anti-IgT [N5P2D2*B4] showing positive membranous and diffuse immunostaining in infected trout kidney. Formalin-fixed paraffin-embedded tissues. 

For Research Use Only.

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