Anti-Natural Killer Cell [H25] Antibody
This monoclonal antibody was generated against HSE-2 cells and recognizes human Natural Killer (NK) cells.
Natural killer cells, large granular lymphocytes (LGL) that develop in the bone marrow, are a critical component of the innate immune system that also play a role in the body’s response to cancer and viral infections. NK cells function by releasing granzymes such as perforin to mediate cytotoxicity through apoptosis or cell lysis. The H25 antibody has been shown to react with a subset of peripheral mononuclear cells with natural killer and killer cell activity as well as all T cell lines tested, but weakly or not at all with cell lines of B, myeloid or erythroid origin.
From a laboratory at Cancer Research Technology, Cancer Research Technology.
Part of The Investigator's Annexe program.
This monoclonal antibody was generated against HSE-2 cells and recognizes human Natural Killer (NK) cells.
Natural killer cells, large granular lymphocytes (LGL) that develop in the bone marrow, are a critical component of the innate immune system that also play a role in the body’s response to cancer and viral infections. NK cells function by releasing granzymes such as perforin to mediate cytotoxicity through apoptosis or cell lysis. The H25 antibody has been shown to react with a subset of peripheral mononuclear cells with natural killer and killer cell activity as well as all T cell lines tested, but weakly or not at all with cell lines of B, myeloid or erythroid origin.
From a laboratory at Cancer Research Technology, Cancer Research Technology.
Part of The Investigator's Annexe program.
| Product Type: | Antibody |
| Antigen: | Natural Killer (NK) cells |
| Molecular Weight: | 96 kDa (determined by IP) |
| Isotype: | IgG1 |
| Clonality: | Monoclonal |
| Clone Name: | H25 |
| Reactivity: | Human |
| Immunogen: | HSE-2 cell line |
| Species Immunized: | Mouse |
| Purification Method: | Protein G |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Tested Applications: | IP, IHC |
| Concentration: | 1 mg/mL |
| Storage: | -20C |
| Shipped: | Cold packs |
- Bai Y, Beverley PC, Knowles RW, Bodmer WF. Two monoclonal antibodies identifying a subset of human peripheral mononuclear cells with natural killer cell activity. Eur J Immunol. 1983 Jul;13(7):521-7. PMID: 6603362
- Körfer A, Kirchner H, Schneekloth C, Bührer C, Wisniewski D, Gulati S, Clarkson B, Knowles R, Poliwoda H, Atzpodien J. Immunophenotypic demonstration of two natural killer surface markers, H25 and H366, on fresh human leukemic cells. Acta Haematol. 1989;82(4):193-6. PMID: 2511721
- Wisniewski D, Strife A, Arlin Z, Knowles R, Lambek C, Gulati S, McHendry B, McKenzie S, Clarkson B. Analysis of the individual and combined reactivities of monoclonal antibodies H25, H366, and MY9 with normal progenitor cells and blast cells from patients with acute myeloblastic leukemia. Leukemia. 1989 Jun;3(6):446-52. PMID: 2657231
- Signore A, Cugini P, Letizia C, Lucia P, Murano G, Pozzilli P. Study of the diurnal variation of human lymphocyte subsets. J Clin Lab Immunol. 1985 May;17(1):25-8. PMID: 2931528
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