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Anti-human Neuropilin-1 b1b2 [clone 1A4; mAb1] Monoclonal Antibody

Invented at University of Tartu

Info

Catalogue Number 160469
Applications IHC IF WB
Antigen/Gene or Protein Targets human Neuropillin-1 b1b2 domain
Synonyms 1A4, mAB1 (mAB1 in studies published in Science)
Reactivity Human
Relevance Monoclonal antibody raised against human NRP-1 b1b2 domain, clone 1A4 (mAB1 in studies published in Science). Partially blocking binding of CendR peptides to b1 domain of NRP-1.

In recent years the relevance of CendR pathway for cellular uptake of biological nanoparticles – viruses - has been demonstrated in several independent studies. A direct role of Neuropilin (NRP) in virus entry has been demonstrated for three viruses, the retrovirus Human T-cell lymphotropic virus type 1 (HTLV-1) and the herpes viruses EBV and CMV5-8. In the case of CMV, NRP-2 acts as a receptor only for specific cell types, while in fibroblasts the virus uses a different molecule for entry.

Viruses that display CendR peptides on their surface are expected to bind a specific CendR binding pocket on the extracellular domain of NRP. Ample evidence obtained with synthetic and phage-displayed CendR peptides shows that they bind to conserved binding pocket in b1 domain of NRP-1. The inventors have developed a monoclonal antibody that specifically interacts with the CendR binding pocket of the b1 domain of Neuropilin-1 and blocks binding of CendR peptides.

This monoclonal antibody has potential applications in the research of SARS-CoV2 in that the antibody is capable to reduce/block the internalisation of SARS-CoV2 into cells, thus to stop viral replication.
Host Mouse
Immunogen Human wild-type Neuropilin-1 CendR b1b2 region
Myeloma Used P3X63Ag8.653
Notes This technology is part of a series of anti- human Neuropilin-1 b1b2 domain mAbs. Two other clones, as well as the expression cosntruct for the immunogen, are available on Ximbio.com (see Related tab).

1A4 - mAB1 Partial CendR blockade

3E7 – mAB2 No CendR blockade

7E8 - mAB3 Complete CendR blockade
Research Area Virology

References

There are 2 reference entries for this reagent.

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References: 2 entries

Cantuti-Castelvetri et al. 2020. Science. 370(6518):856-860. PMID: 33082293.

Daly et al. 2020. Science. 370(6518):861-865. PMID: 33082294.


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References: 2 entries

Cantuti-Castelvetri et al. 2020. Science. 370(6518):856-860. PMID: 33082293.

Daly et al. 2020. Science. 370(6518):861-865. PMID: 33082294.


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Inventor Information

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