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Mice expressing human Menkes gene ATP7A

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Antigen/Gene or Protein Targets ATP7A
Disease Keywords Menkes Disease
Model Transgenic
Relevance The Menkes protein (ATP7A) is defective in the Cu deficiency disorder Menkes disease and is an important contributor to the maintenance of physiological Cu homeostasis. These Transgenic mice expressing the human Menkes gene ATP7A from chicken β-actin composite promoter provide a way to investigate more fully the role of ATP7A. The transgenic mice express ATP7A in lung, heart, liver, kidney, small intestine, and brain but display no overt phenotype resulting from expression of the human protein. PMID: 16397091.
Production Details The mammalian expression vector pcDNA3 was modified by replacing its origin of replication with that from pWSK29. Furthermore, the cytomegalovirus (CMV) promoter was replaced with the CAG promoter to generate the plasmid pCMB336. The myc-tagged ATP7A cDNA was inserted into the EcoRV/XhoI restriction enzyme sites of pCMB336 generating plasmid pCMB344. An 8.8-kb PvuI/StuI fragment containing CAG-ATP7A-bovine growth hormone poly(A) signal was used at 2 ng/ml for pronuclear microinjection. PMID: 16397091.
Strain C57BL/6
Research Area Other

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References: 1 entry

Ke et al. 2006. Am J Physiol Regul Integr Comp Physiol. 290(5):R1460-7. PMID: 16397091.


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References: 1 entry

Ke et al. 2006. Am J Physiol Regul Integr Comp Physiol. 290(5):R1460-7. PMID: 16397091.


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