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Monoclonal Antibodies

Anti-uncarboxylated osteocalcin (ucOC) [Fab-16] recombinant antibody

Invented by Kaisa Ivaska-Papaioannou , Urpo Lamminmäki
Invented at University Of Turku
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Image thumbnail for Anti-uncarboxylated osteocalcin (ucOC) [Fab-16] recombinant antibody
Image thumbnail for Anti-uncarboxylated osteocalcin (ucOC) [Fab-16] recombinant antibody
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From Arponen et al Calcified Tissue International volume 107, pages 529–542(2020). Characterization of the recombinant Fab-AP–antibodies. The binding site in ucOC was determined for Fab-AP13 (black), Fab-AP2 (white), Fab-AP16 (gray), Fab-AP19 (white with stripes) and MAb-2H9 (gray with stripes) by testing biotinylated linear human osteocalcin peptides.
From Arponen et al Calcified Tissue International volume 107, pages 529–542(2020). Conversion from scFv-AP to Fab-AP improved the thermostability of the antibodies. Thermostability of the recombinant antibodies Fab-AP (open) and scFv-AP (solid) was tested by heating the antibodies Fab-AP13 (a), Fab-AP2 (b), Fab-AP16 (c), and Fab-AP19 (d) from 40 °C up to 71 °C. Reference antibody was kept on ice during the heating process and set as 100% and Tm was assessed when 50% of immunoreactivity was lost (gray dot line).
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Image thumbnail for Anti-uncarboxylated osteocalcin (ucOC) [Fab-16] recombinant antibody Image thumbnail for Anti-uncarboxylated osteocalcin (ucOC) [Fab-16] recombinant antibody
Catalogue Number 157722
Applications IF Fn
Antigen/Gene or Protein Targets osteocalcin - partially carboxylated with Glu at position 17
Synonyms Uncarboxylated osteocalcin, uc-Osteocalcin, uc-OC, bone gamma-carboxyglutamic acid-containing protein, BGLAP
Reactivity Human
Relevance Recombinant antibody binding to partially carboxylated osteocalcin with Glu at position 17
Host Human
Immunogen Human full-length ucOC peptide (1–49, Glu at positions 17, 21 and 24; C23–C29 bridge)
Subclass IgG1 kappa
Notes Adapted from Arponen et al Calcified Tissue International volume 107, pages 529–542(2020).
These ucOC-specific recombinant antibodies were selected from an antibody library by phage display. Four candidates were characterized - all linked in the above "Related" tab.
Research Area Diabetes, Metabolism, Other

References

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References: 1 entry

Arponen et al. 2020. Calcif Tissue Int. 107(6):529-542. PMID: 32839842.

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References: 1 entry

Arponen et al. 2020. Calcif Tissue Int. 107(6):529-542. PMID: 32839842.

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Inventor Information

Inventors

Kaisa Ivaska-Papaioannou

Kaisa Ivaska-Papaioannou

Urpo Lamminmäki

Urpo Lamminmäki

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Invented at

University Of Turku
University Of Turku

10 reagents listed on Ximbio