Anti-CD45 [C363.16A] monoclonal antibody
Invented at Yale University
- Datasheet
- References (7)
- Inventor Info
Info
| Catalogue Number | 155241 |
| Applications | FACS IF IP WB |
| Antigen/Gene or Protein Targets | CD45 |
| Synonyms | YCD45R-1 |
| Reactivity | Mouse |
| Relevance |
Monoclonal antibody directed against CD45RB isoform of CD45. Background and Research Application CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. CD45 regulates Src-family kinases, integrin-mediated signal transduction pathways and can suppress JAK kinases, acting as a negative regulator of cytokine receptor signalling. It plays a critical role in TCR and BCR signalling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. CD45RB is expressed in microglia and inflammatory cells. In addition to involvement in immunoreceptor signalling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is involved in DNA fragmentation during apoptosis. |
| Host | Rat |
| Immunogen | cloned T cell lines belonging to the helper T cell (Th2) subset, final boost was given with helper/Th2 clone D10 |
| Immunogen UniProt ID | P08575 |
| Subclass | IgG |
| Molecular Weight (kDa) | 190 kDa and 220 kDa |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Concentration 1mg/ml as standard |
| Research Area | Immunology |
References: 7 entries
Shao et al. 2020. J Immunol. 204(1):37-48. PMID: 31757864.
Garo et al. 2019. Cell Rep. 28(13):3353-3366.e5. PMID: 31553906.
Timilshina et al. 2019. Cell Rep. 27(10):2948-2961.e7. PMID: 31167140.
Imbratta et al. 2019. Sci Rep. 9(1):6135. PMID: 30992496.
Lyons et al. 2018. PLoS Biol. 16(3):e2002417. PMID: 29596476.
Lyons et al. 2018. Sci Signal. 11(519):. PMID: 29487189.
Bottomly et al. 1989. Eur J Immunol. 19(4):617-23. PMID: 2567241.
Add a reference
References: 7 entries
Shao et al. 2020. J Immunol. 204(1):37-48. PMID: 31757864.
Garo et al. 2019. Cell Rep. 28(13):3353-3366.e5. PMID: 31553906.
Timilshina et al. 2019. Cell Rep. 27(10):2948-2961.e7. PMID: 31167140.
Imbratta et al. 2019. Sci Rep. 9(1):6135. PMID: 30992496.
Lyons et al. 2018. PLoS Biol. 16(3):e2002417. PMID: 29596476.
Lyons et al. 2018. Sci Signal. 11(519):. PMID: 29487189.
Bottomly et al. 1989. Eur J Immunol. 19(4):617-23. PMID: 2567241.
Add a reference
