MEF2BD83V Mouse
Invented at The Trustees of Columbia University in the City of New York
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- References (2)
- Inventor Info
Info
| Catalogue Number | 154096 |
| Antigen/Gene or Protein Targets | MEF2B |
| Disease Keywords | B-Cell Lymphoma |
| Synonyms | Myocyte Enhancer Factor 2B, Serum Response Factor-Like Protein 2, RSRFR2, XMEF2 |
| Model | Knock-In |
| Relevance | Diffuse large B-Cell Lymphoma (DLBCL) and Follicular Lymphoma (FL) are the two most common forms of mature B cell lymphoid neoplams, accounting for over 50% of all diagnoses. Mutant MEF2B (MEF2BD83V) is the most common lymphoma associated oncogene. MEF2BD83V expression in mice leads to GC enlargement and lymphoma development, a phenotype that becomes fully penetrant in combination with BCL2 de-regulation, an event associated with human MEF2B mutations. Expression of the mutant MEF2BD38V allele was induced in germinal centre- derived B cells by crossing with Cγ1-Cre mice. |
| Production Details | The conditional Mef2bstopD83V allele was generated by introducing a single nucleotide change by site directed mutagenesis leading to the D83V amino-acid change in exon 3. The neomycin-resistance marker followed by a triple-polyA, flanked by two loxP sites was introduced upstream the exon 3 carrying the D83V mutation. Correctly targeted mouse ES cells were injected into blastocysts derived from C57BL/6 mice to generate chimeras. |
| Conditional Description | Conditional Knockin mice that express mutant MEF2BD38V upon recombination by Cre-recombinase |
| Strain | C57BL/6 |
| Mouse Genetic Background/Cross History | 129/Sv-C57Bl/6 mixed |
| Zygosity | Homozygous |
| Research Area | Cancer, Immunology |
References: 2 entries
Brescia et al. 2018. Cancer Cell. 34(3):453-465.e9. PMID: 30205047.
MEF2B Instructs Germinal Center Development and Acts as an Oncogene in B Cell Lymphomagenesis.
Europe PMC ID: 30205047
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References: 2 entries
Brescia et al. 2018. Cancer Cell. 34(3):453-465.e9. PMID: 30205047.
MEF2B Instructs Germinal Center Development and Acts as an Oncogene in B Cell Lymphomagenesis.
Add a reference
